Human PHPT1 Recombinant Protein (RPPB2110)
- SKU:
- RPPB2110
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- PHPT1
- Synonyms:
- PHP14
- CGI-202
- HSPC141
- Phosphohistidine Phosphatase 1
- Source:
- Escherichia Coli
- Uniprot:
- Q9NRX4
Description
| Product Name: | Human PHPT1 Recombinant Protein |
| Product Code: | RPPB2110 |
| Size: | 10µg |
| Species: | Human |
| Target: | PHPT1 |
| Synonyms: | PHP14, CGI-202, HSPC141, Phosphohistidine Phosphatase 1, Phosphohistidine Phosphatase 14kDa, Protein janus-A homolog, Sex-regulated protein Janus-a. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The PHPT1 protein solution (0.5mg/ml) is formulated in 20mM Tris-HCl buffer (pH8.0), 0.2M NaCl, 2mM DTT and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MAVADLALIP DVDIDSDGVF KYVLIRVHSA PRSGAPAAES KEIVRGYKWA EYHADIYDKV SGDMQKQGCD CECLGGGRIS HQSQDKKIHV YGYSMAYGPA QHAISTEKIK AKYPDYEVTW ANDGY |
| Biological Activity: | Specific activity is >120 units/mg, and is defined as the amount of enzyme that hydrolyze 1nmol f p-nitrophenyl phosphate per minute at pH7.5 at 37°C. |
PHPT1 is a member of the Janus protein familyand is 125 amino acid long. PHPT1 appears as a monomer in the cytoplasm and is an EDTA-insensitive phosphohistidine phosphatase. Overexpression of PHPT1 resolts in specific phosphohistidine phosphatase activity towards phosphopeptide I, with no activity detected towards phosphotyrosine, phosphothreonine and phosphoserine peptides.
PHPT1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 145 amino acids (1-125a.a.) and having a molecular mass of 15.9kDa.PHPT1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PHPT1: a phosphohistidine phosphatase. Present preferentially in heart and skeletal muscle. Insensitive to inhibition by okadaic acid and EDTA. |
| UniProt Protein Details: | Protein type:Carbohydrate Metabolism - fructose and mannose; Phosphatase; Cofactor and Vitamin Metabolism - riboflavin; EC 3.1.3.-; Motility/polarity/chemotaxis; Cofactor and Vitamin Metabolism - thiamine Chromosomal Location of Human Ortholog: 9q34.3 Cellular Component: cytosol Molecular Function:phosphohistidine phosphatase activity; calcium channel inhibitor activity; phosphoprotein phosphatase activity Biological Process: negative regulation of lyase activity; negative regulation of T cell receptor signaling pathway; protein amino acid dephosphorylation |
| NCBI Summary: | This gene encodes an enzyme that catalyzes the reversible dephosphorylation of histidine residues in proteins. It may be involved in the dephosphorylation of G-beta and ATP citrate lyase and in negatively regulating CD4 T lymphocytes by dephosphorylation and inhibition of KCa3.1 channels. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013] |
| UniProt Code: | Q9NRX4 |
| NCBI GenInfo Identifier: | 25008934 |
| NCBI Gene ID: | 29085 |
| NCBI Accession: | Q9NRX4.1 |
| UniProt Secondary Accession: | Q9NRX4,Q9H0Y3, B1AMX0, B1AMX1, |
| UniProt Related Accession: | Q9NRX4 |
| Molecular Weight: | 125 |
| NCBI Full Name: | 14 kDa phosphohistidine phosphatase |
| NCBI Synonym Full Names: | phosphohistidine phosphatase 1 |
| NCBI Official Symbol: | PHPT1 |
| NCBI Official Synonym Symbols: | PHP14; CGI-202; HSPC141; HEL-S-132P |
| NCBI Protein Information: | 14 kDa phosphohistidine phosphatase; protein janus-A homolog; sex-regulated protein janus-a; epididymis secretory sperm binding protein Li 132P |
| UniProt Protein Name: | 14 kDa phosphohistidine phosphatase |
| UniProt Synonym Protein Names: | Phosphohistidine phosphatase 1; Protein janus-A homolog |
| Protein Family: | 14 kDa phosphohistidine phosphatase |
| UniProt Gene Name: | PHPT1 |
| UniProt Entry Name: | PHP14_HUMAN |
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