Human PHOSPHO2 Recombinant Protein (RPPB2108)
- SKU:
- RPPB2108
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- PHOSPHO2
- Synonyms:
- Pyridoxal phosphate phosphatase PHOSPHO2
- PHOSPHO2
- Source:
- Escherichia Coli
- Uniprot:
- Q8TCD6
Description
| Product Name: | Human PHOSPHO2 Recombinant Protein |
| Product Code: | RPPB2108 |
| Size: | 10µg |
| Species: | Human |
| Target: | PHOSPHO2 |
| Synonyms: | Pyridoxal phosphate phosphatase PHOSPHO2, PHOSPHO2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The PHOSPHO2 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 10% glycerol and 0.1M NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMKILLV FDFDNTIIDD NSDTWIVQCA PNKKLPIELR DSYRKGFWTE FMGRVFKYLG DKGVREHEMK RAVTSLPFTP GMVELFNFIR KNKDKFDCII ISDSNSVFID WVLEAASFHD IFDKVFTNPA AFNSNGHLTV ENYHTHSCNR CPKNLCKKVV LIEFVDKQLQ QGVNYTQIVY IGDGGNDVCP VTFLKNDDVA MPRKGYTLQK TLSRMSQNLE PMEYSVVVWS SGVDIISHLQ FLIKD |
Pyridoxal phosphate phosphatase PHOSPHO2, orphan 2 (PHOSPHO2) is a member of the haloacid dehalogenase (HAD) superfamily. Phosphatase has an elevated activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). PHOSPHO 1, a phosphoethanolamine/phosphocholine phosphatase, is upregulated in mineralizing cells and is believed to be implicated in the production of inorganic phosphate for bone mineralization. PHOSPHO2 is a recognized phosphatase sharing a 42% sequence identity with PHOSPHO1. PHOSPHO1 and PHOSPHO2 are especially similar, however surprisingly recombinant PHOSPHO2 hydrolyses phosphoethanolamine and phosphocholine comparatively inadequately.
PHOSPHO2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 265 amino acids (1-241) and having a molecular mass of 30.3kDa.PHOSPHO2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PHOSPHO2: Phosphatase that has high activity toward pyridoxal 5'- phosphate (PLP). Also active at much lower level toward pyrophosphate, phosphoethanolamine (PEA), phosphocholine (PCho), phospho-l-tyrosine, fructose-6-phosphate, p-nitrophenyl phosphate, and h-glycerophosphate. Belongs to the HAD-like hydrolase superfamily. PHOSPHO family. |
| UniProt Protein Details: | Protein type:EC 3.1.3.74; Phosphatase Chromosomal Location of Human Ortholog: 2q31.1 Molecular Function:phosphoric monoester hydrolase activity; protein binding |
| UniProt Code: | Q8TCD6 |
| NCBI GenInfo Identifier: | 74730590 |
| NCBI Gene ID: | 493911 |
| NCBI Accession: | Q8TCD6.1 |
| UniProt Secondary Accession: | Q8TCD6,B2RC30, D3DPC7, |
| UniProt Related Accession: | Q8TCD6 |
| Molecular Weight: | 28kDa |
| NCBI Full Name: | Pyridoxal phosphate phosphatase PHOSPHO2 |
| NCBI Synonym Full Names: | phosphatase, orphan 2 |
| NCBI Official Symbol: | PHOSPHO2 |
| NCBI Protein Information: | pyridoxal phosphate phosphatase PHOSPHO2 |
| UniProt Protein Name: | Pyridoxal phosphate phosphatase PHOSPHO2 |
| Protein Family: | Probable phosphatase |
| UniProt Gene Name: | PHOSPHO2 |
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