Human PGRP-S (Peptidoglycan Recognition Protein-S) ELISA Kit
The Human PGRP-S (Peptidoglycan Recognition Protein-S) ELISA Kit is a specialized assay designed for the precise measurement of PGRP-S levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reliable results suitable for a variety of research applications.PGRP-S is a key protein involved in the recognition of peptidoglycan, a component of bacterial cell walls. It plays a critical role in the innate immune response, serving as a sensor for bacterial infections and activating immune defenses.
The detection of PGRP-S levels can provide valuable insights into immune responses and the development of infectious diseases.This ELISA kit is an essential tool for researchers studying innate immunity, bacterial infections, and immune responses. With its high performance and ease of use, the Human PGRP-S ELISA Kit from Assay Genie is a valuable resource for advancing research in immunology and infectious diseases.
Product Name:
Human PGRP-S (Peptidoglycan Recognition Protein-S) ELISA Kit
SKU:
HUES02920
Target:
Human PGRP-S (Peptidoglycan Recognition Protein-S)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PGRP-S. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PGRP-S and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PGRP-S, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PGRP-S. You can calculate the concentration of Human PGRP-S in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-108
93-109
84-97
Average (%)
101
99
91
1:4
Range (%)
87-102
86-99
89-100
Average (%)
94
92
94
1:8
Range (%)
92-108
82-92
85-96
Average (%)
99
87
90
1:16
Range (%)
87-97
86-100
86-99
Average (%)
92
93
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-110
100
EDTA plasma (n=5)
83-97
90
Cell culture media (n=5)
86-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.93
2.75
8.01
1.01
2.9
8.66
Standard deviation
0.05
0.16
0.27
0.05
0.16
0.47
C V (%)
5.38
5.82
3.37
4.95
5.52
5.43
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PGRP-S concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PGRP-S in samples. No significant cross-reactivity or interference between Human PGRP-S and analogues was observed.
