Human PGK2 Recombinant Protein (RPPB2605)
- SKU:
- RPPB2605
- Product type:
- Recombinant Protein
- Size:
- 5ug
- Species:
- Human
- Target:
- PGK2
- Synonyms:
- Phosphoglycerate kinase 2
- dJ417L202
- PGKB
- PGKPSS
- Source:
- Escherichia Coli
- Uniprot:
- P07205
Description
| Product Name: | Human PGK2 Recombinant Protein |
| Product Code: | RPPB2605 |
| Size: | 5µg |
| Species: | Human |
| Target: | PGK2 |
| Synonyms: | Phosphoglycerate kinase 2, dJ417L20.2, PGKB, PGKPSS, Phosphoglycerate kinase, testis specific |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The PGK2 solution (0.5mg/ml) contains 20% glycerol, 20mM Tris-HCl buffer (pH 8.0), 1mM DTT and 0.1M NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MSLSKKLTLD KLDVRGKRVI MRVDFNVPMK KNQITNNQRI KASIPSIKYC LDNGAKAVVL MSHLGRPDGV PMPDKYSLAP VAVELKSLLG KDVLFLKDCV GAEVEKACAN PAPGSVILLE NLRFHVEEEG KGQDPSGKKI KAEPDKIEAF RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPHKASGFLM KKELDYFAKA LENPVRPFLA ILGGAKVADK IQLIKNMLDK VNEMIIGGGM AYTFLKVLNN MEIGASLFDE EGAKIVKDIM AKAQKNGVRI TFPVDFVTGD KFDENAQVGK ATVASGISPG WMGLDCGPES NKNHAQVVAQ ARLIVWNGPL GVFEWDAFAK GTKALMDEIV KATSKGCITV IGGGDTATCC AKWNTEDKVS HVSTGGGASL ELLEGKILPG VEALSNM |
| Biological Activity: | Specific activity > 500unit/mg. One unit will convert 1 umole of 1,3-Bisphosphoglycerate to 3-PGA per minute at pH 8.0 at 37?C. |
PGK2, also known as Phosphoglycerate kinase 2, is a testis-specific form of phosphoglycerate kinase.Originally, it was assumed that PGK2was a pseudogene. However, nowadays it is known that this protein is a functional phosphoglycerate kinase. During the Embden-Meyerhof-Parnas pathway of glycolysis, in thelater stages of spermatogenesis, the protein catalyses the reversible conversion of 1, 3- bisphosphoglycerate to 3-phosphoglycerate.
PGK2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 437 amino acids (1-417a.a.) and having a molecular mass of 46.9kDa.PGK2 is fused to a 20 amino acid His tag at N-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PGK2: This gene is intronless, arose via retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis. Initially assumed to be a pseudogene, the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis.[provided by RefSeq, May 2010] |
| UniProt Protein Details: | Protein type:Carbohydrate Metabolism - glycolysis and gluconeogenesis; Kinase, other; EC 2.7.2.3 Chromosomal Location of Human Ortholog: 6p12.3 Cellular Component: nucleus Molecular Function:ATP binding; phosphoglycerate kinase activity Biological Process: phosphorylation |
| NCBI Summary: | This gene is intronless, arose via retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis. Initially assumed to be a pseudogene, the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis.[provided by RefSeq, May 2010] |
| UniProt Code: | P07205 |
| NCBI GenInfo Identifier: | 21264485 |
| NCBI Gene ID: | 5232 |
| NCBI Accession: | P07205.3 |
| UniProt Secondary Accession: | P07205,Q9H107, B2R6Y8, |
| UniProt Related Accession: | P07205 |
| Molecular Weight: | 44,796 Da |
| NCBI Full Name: | Phosphoglycerate kinase 2 |
| NCBI Synonym Full Names: | phosphoglycerate kinase 2 |
| NCBI Official Symbol: | PGK2 |
| NCBI Official Synonym Symbols: | PGKB; PGKPS; HEL-S-272; dJ417L20.2 |
| NCBI Protein Information: | phosphoglycerate kinase 2 |
| UniProt Protein Name: | Phosphoglycerate kinase 2 |
| UniProt Synonym Protein Names: | Phosphoglycerate kinase, testis specific |
| Protein Family: | Phosphoglycerate kinase |
| UniProt Gene Name: | PGK2 |
| UniProt Entry Name: | PGK2_HUMAN |
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