Human PFN2 Recombinant Protein (RPPB4235)

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SKU:
RPPB4235
Product type:
Recombinant Protein
Size:
10ug
Species:
Human
Target:
PFN2
Synonyms:
Profilin-II
Synonyms:
PFN2
Synonyms:
Profilin-2
Synonyms:
PFL
Source:
Escherichia Coli
Uniprot:
P35080
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Description

Product Name:Human PFN2 Recombinant Protein
Product Code:RPPB4235
Size:10µg
Species:Human
Target:PFN2
Synonyms:Profilin-II, PFN2, Profilin-2, PFL, D3S1319E.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:PFN2 protein solution (1mg/ml) containing 20mM Tris-HCl pH-8, 1mM DTT and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MAGWQSYVDN LMCDGCCQEA AIVGYCDAKY VWAATAGGVF QSITPIEIDM IVGKDREGFF TNGLALGAKK CSVIRDSLYV DGDCTMDIRT KSQGGEPTYN VAVGRAGRVL VFVMGKEGVH GGGLNKKAYS MAKYLRDSGF

PFN2 is a ubiquitous actin monomer-binding protein which is part of the profilin family. PFN2 regulates actin polymerization in response to extra cellular signals. PFN2 binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, while it increases it at low concentrations. PFN2 binds to PIP2, it inhibits the formation of IP3 and DG.

PFN2 Human Recombinant produced in E.Coli is a single, non-glycosylated,polypeptide chain containing 160 amino acids (1-140 a.a.) and having a molecular mass of 17.2 kDa. PFN2 protein is fused to a 20 amino acid His-Tag at N-terminus and purified by standard chromatography.

UniProt Protein Function:profilin 2: Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Belongs to the profilin family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Actin-binding

Chromosomal Location of Human Ortholog: 3q25.1

Cellular Component: cytoskeleton; cytoplasm; terminal button

Molecular Function:actin monomer binding; protein binding; phosphatidylinositol-4,5-bisphosphate binding; adenyl-nucleotide exchange factor activity

Biological Process: positive regulation of actin filament polymerization; positive regulation of ATPase activity; negative regulation of actin filament polymerization; positive regulation of stress fiber formation; positive regulation of actin filament bundle formation; actin cytoskeleton organization and biogenesis; positive regulation of peptidyl-serine phosphorylation

NCBI Summary:The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. There are two alternatively spliced transcript variants encoding different isoforms described for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P35080
NCBI GenInfo Identifier:20178322
NCBI Gene ID:5217
NCBI Accession:P35080.3
UniProt Secondary Accession:P35080,Q4VBQ4, Q8WVF9, Q9HBK2, B2R4C8, D3DNI4,
UniProt Related Accession:P35080
Molecular Weight:140
NCBI Full Name:Profilin-2
NCBI Synonym Full Names:profilin 2
NCBI Official Symbol:PFN2  
NCBI Official Synonym Symbols:PFL; D3S1319E  
NCBI Protein Information:profilin-2; profilin II
UniProt Protein Name:Profilin-2
UniProt Synonym Protein Names:Profilin II
UniProt Gene Name:PFN2  
UniProt Entry Name:PROF2_HUMAN
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