Human PDGF-BB (Platelet Derived Growth Factor-BB) ELISA Kit
The Human PDGF-BB (Platelet-Derived Growth Factor BB) ELISA Kit is specifically designed for the precise quantification of PDGF-BB levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing consistent and accurate results for a variety of research applications.PDGF-BB is a key growth factor that plays a critical role in regulating cell growth, proliferation, and differentiation. It is essential for wound healing, tissue repair, and maintaining normal cell function. Dysregulation of PDGF-BB expression has been implicated in various diseases such as cancer, fibrosis, and cardiovascular disorders, making it a valuable biomarker for investigating disease mechanisms and developing potential therapeutic interventions.
By utilizing the Human PDGF-BB ELISA Kit, researchers can confidently measure PDGF-BB levels in biological samples with precision, facilitating in-depth studies on the role of PDGF-BB in health and disease. This kit is a valuable tool for advancing scientific understanding and driving progress in biomedical research.
Product Name:
Human PDGF-BB (Platelet Derived Growth Factor-BB) ELISA Kit
SKU:
HUES02588
Target:
Human PDGF-BB (Platelet Derived Growth Factor-BB)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PDGF-BB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PDGF-BB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PDGF-BB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PDGF-BB. You can calculate the concentration of Human PDGF-BB in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-106
85-97
89-103
Average (%)
100
91
96
1:4
Range (%)
87-99
80-91
89-101
Average (%)
93
85
95
1:8
Range (%)
86-99
83-97
89-101
Average (%)
93
90
94
1:16
Range (%)
89-105
86-102
83-97
Average (%)
96
93
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
102
EDTA plasma (n=5)
85-101
92
Cell culture media (n=5)
87-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
108.61
262.35
849.02
105.51
269.64
922.05
Standard deviation
7.16
12.8
34.56
6.9
13.0
39.37
C V (%)
6.59
4.88
4.07
6.54
4.82
4.27
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PDGF-BB concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PDGF-BB in samples. No significant cross-reactivity or interference between Human PDGF-BB and analogues was observed.
