Human PDGF-AB (Platelet Derived Growth Factor AB) ELISA Kit
The Human PDGF (Platelet-Derived Growth Factor) AB ELISA Kit is specifically designed for the precise measurement of PDGF levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.PDGF is a key growth factor known for its role in promoting cell growth, proliferation, and differentiation. It plays a crucial function in wound healing, tissue repair, and development of various organs.
Dysregulation of PDGF signaling has been implicated in conditions such as cancer, fibrosis, and vascular diseases, highlighting its importance as a biomarker for disease progression and potential therapeutic targets.With reliable performance and easy-to-follow protocols, the Human PDGF AB ELISA Kit from Assay Genie is an essential tool for researchers investigating the role of PDGF in health and disease, providing valuable insights into mechanisms of growth regulation and potential treatment strategies.
Product Name:
Human PDGF-AB (Platelet Derived Growth Factor AB) ELISA Kit
SKU:
HUES02587
Target:
Human PDGF-AB (Platelet Derived Growth Factor AB)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PDGF-AB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PDGF-AB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PDGF-AB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PDGF-AB. You can calculate the concentration of Human PDGF-AB in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-103
93-110
99-115
Average (%)
97
100
105
1:4
Range (%)
88-102
86-98
82-95
Average (%)
95
91
88
1:8
Range (%)
91-106
88-99
85-99
Average (%)
98
93
91
1:16
Range (%)
88-104
86-101
83-95
Average (%)
95
92
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-109
101
EDTA plasma (n=5)
86-98
91
Cell culture media (n=5)
95-108
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
97.88
260.1
731.06
88.26
235.21
750.26
Standard deviation
6.67
14.98
34.87
5.6
11.93
31.74
C V (%)
6.81
5.76
4.77
6.34
5.07
4.23
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PDGF-AB concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PDGF-AB in samples. No significant cross-reactivity or interference between Human PDGF-AB and analogues was observed.
