Human PDE6D Recombinant Protein (RPPB2078)
- SKU:
- RPPB2078
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- PDE6D
- Synonyms:
- Retinal rod rhodopsin-sensitive cGMP 3'
- 5'-cyclic phosphodiesterase subunit delta
- Phosphodiesterase 6D cGMP-Specific Rod Delta
- GMP-PDE delta
- Source:
- Escherichia Coli
- Uniprot:
- O43924
Description
| Product Name: | Human PDE6D Recombinant Protein |
| Product Code: | RPPB2078 |
| Size: | 20µg |
| Species: | Human |
| Target: | PDE6D |
| Synonyms: | Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta, Phosphodiesterase 6D cGMP-Specific Rod Delta, GMP-PDE delta, Protein p17, PDE6D, PDED. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The PDE6D protein solution contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol, 1mM DTT and 100mM NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MSAKDERARE ILRGFKLNWM NLRDAETGKI LWQGTEDLSV PGVEHEARVP KKILKCKAVS RELNFSSTEQ MEKFRLEQKV YFKGQCLEEW FFEFGFVIPN STNTWQSLIE AAPESQMMPA SVLTGNVIIE TKFFDDDLLV STSRVRLFYV GSHHHHHH |
Human PDE6D was initially identified as a fourth subunit of rod-specific cGMP phosphodiesterase, PDE6. PDE6D is an Oligomer composed of two catalytic chains (alpha and beta), an inhibitory chain (gamma) and the delta chain. PDE6D interacts with RPGR, ARL2 and ARL3. The catalytically active PDE6 is a heterodimer (?Beta) that is controlled by two inhibitory ? subunits. Since PDE6D does not change the catalytic properties of PDE?Beta, its function is still unanswered. PDE6D is expressed specifically in the retina.
PDE6D produced in E.Coli is a single, non-glycosylated polypeptide chain containing 158 amino acids (1-150 a.a.) and having a molecular mass of 18.4kDa. PDE6D is fused to an 8 amino acids His Tag at C-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PDE6D: Acts as a GTP specific dissociation inhibitor (GDI). Increases the affinity of ARL3 for GTP by several orders of magnitude and does so by decreasing the nucleotide dissociation rate. Stabilizes Arl3-GTP by decreasing the nucleotide dissociation. Belongs to the PDE6D/unc-119 family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 2q35-q36 Cellular Component: cytoplasmic vesicle; cytosol Molecular Function:GTPase inhibitor activity; protein binding; Rab GTPase binding Disease: Joubert Syndrome 22 |
| NCBI Summary: | This gene encodes the delta subunit of rod-specific photoreceptor phosphodiesterase (PDE), a key enzyme in the phototransduction cascade. A similar protein in cow functions in solubilizing membrane-bound PDE. In addition to its role in the PDE complex, the encoded protein is thought to bind to prenyl groups of proteins to target them to subcellular organelles called cilia. Mutations in this gene are associated with Joubert syndrome-22. Alternative splicing results in multiple splice variants. [provided by RefSeq, Mar 2014] |
| UniProt Code: | O43924 |
| NCBI GenInfo Identifier: | 5921809 |
| NCBI Gene ID: | 5147 |
| NCBI Accession: | O43924.1 |
| UniProt Secondary Accession: | O43924,O43250, |
| UniProt Related Accession: | O43924 |
| Molecular Weight: | 17,420 Da |
| NCBI Full Name: | Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta |
| NCBI Synonym Full Names: | phosphodiesterase 6D |
| NCBI Official Symbol: | PDE6D |
| NCBI Official Synonym Symbols: | PDED; JBTS22 |
| NCBI Protein Information: | retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta |
| UniProt Protein Name: | Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta |
| UniProt Synonym Protein Names: | Protein p17 |
| Protein Family: | Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase |
| UniProt Gene Name: | PDE6D |
| UniProt Entry Name: | PDE6D_HUMAN |
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