The Human PCIII (Procollagen III) ELISA Kit is specifically designed for the precise measurement of Procollagen III levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and consistent results, making it perfect for various research applications.Procollagen III is an important precursor protein involved in collagen synthesis, playing a key role in maintaining skin elasticity and overall tissue structure.
Imbalances in Procollagen III levels have been linked to conditions such as liver fibrosis, arthritis, and dermatological disorders, making it a valuable biomarker for understanding these diseases and exploring potential therapies.This ELISA kit provides researchers with a reliable tool for studying Procollagen III levels in human samples, offering a deep insight into the underlying mechanisms of these conditions and paving the way for innovative treatment strategies.
Product Name:
Human PCIII (Procollagen III) ELISA Kit
SKU:
HUES01449
Target:
Human PCIII (Procollagen III)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PCⅢ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PCⅢ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PCⅢ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PCⅢ. You can calculate the concentration of Human PCⅢ in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-113
87-103
92-106
Average (%)
106
94
97
1:4
Range (%)
96-109
85-96
98-112
Average (%)
103
90
104
1:8
Range (%)
99-116
84-97
98-114
Average (%)
107
89
104
1:16
Range (%)
97-114
89-101
95-111
Average (%)
104
94
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-104
95
EDTA plasma (n=5)
92-108
98
Cell culture media (n=5)
95-108
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.3
4.1
0.5
1.3
3.9
Standard deviation
0.03
0.08
0.14
0.03
0.06
0.16
C V (%)
6
6.15
3.41
6.0
4.62
4.1
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PCⅢ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PCⅢ in samples. No significant cross-reactivity or interference between Human PCⅢ and analogues was observed.
