Human PARK7 Recombinant Protein (RPPB4187)

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SKU:
RPPB4187
Product type:
Recombinant Protein
Size:
20ug
Species:
Human
Target:
PARK7
Synonyms:
Protein DJ-1
Synonyms:
Oncogene DJ1
Synonyms:
Parkinson disease protein 7
Synonyms:
PARK7
Source:
Escherichia Coli
Uniprot:
Q99497
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Description

Product Name:Human PARK7 Recombinant Protein
Product Code:RPPB4187
Size:20µg
Species:Human
Target:PARK7
Synonyms:Protein DJ-1, Oncogene DJ1, Parkinson disease protein 7, PARK7, DJ1, DJ-1, FLJ27376.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The protein solution (1mg/ml) contains 20mM Tris-HCl pH-8 and 20% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMASK RALVILAKGA EEMETVIPVD VMRRAGIKVT VAGLAGKDPV QCSRDVVICP DASLEDAKKE GPYDVVVLPG GNLGAQNLSE SAAVKEILKE QENRKGLIAA ICAGPTALLA HEIGFGSKVT THPLAKDKMM NGGHYTYSEN RVEKDGLILT SRGPGTSFEF ALAIVEALNG KEVAAQVKAP LVLKD

The PARK7 is a ubiquitously expressed protein involved in various cellular processes including spermatogenesis and fertilization, cancer, RNA-binding, androgen-receptor signaling and oxidative stress. Mutations in the PARK7 are the cause of autosomal recessive early-onset Parkinson’s disease 7 (Park7).

PARK7 Human Recombinant fused to an N-terminal 36 aa His-Tag, produced in E.Coli is a single, non-glycosylated polypeptide chain (aa 1-189) containing 225 amino acids and having a molecular mass of 24 kDa.

UniProt Protein Function:Protein and nucleotide deglycase that catalyzes the deglycation of the Maillard adducts formed between amino groups of proteins or nucleotides and reactive carbonyl groups of glyoxals (PubMed:25416785, PubMed:28596309). Thus, functions as a protein deglycase that repairs methylglyoxal- and glyoxal-glycated proteins, and releases repaired proteins and lactate or glycolate, respectively. Deglycates cysteine, arginine and lysine residues in proteins, and thus reactivates these proteins by reversing glycation by glyoxals. Acts on early glycation intermediates (hemithioacetals and aminocarbinols), preventing the formation of advanced glycation endproducts (AGE) that cause irreversible damage (PubMed:25416785, PubMed:28013050, PubMed:26995087). Also functions as a nucleotide deglycase able to repair glycated guanine in the free nucleotide pool (GTP, GDP, GMP, dGTP) and in DNA and RNA. Is thus involved in a major nucleotide repair system named guanine glycation repair (GG repair), dedicated to reversing methylglyoxal and glyoxal damage via nucleotide sanitization and direct nucleic acid repair (PubMed:28596309). Also displays an apparent glyoxalase activity that in fact reflects its deglycase activity (PubMed:22523093). Plays an important role in cell protection against oxidative stress and cell death acting as oxidative stress sensor and redox-sensitive chaperone and protease; functions probably related to its primary function (PubMed:17015834, PubMed:20304780, PubMed:18711745, PubMed:12796482, PubMed:19229105, PubMed:25416785, PubMed:26995087). It is involved in neuroprotective mechanisms like the stabilization of NFE2L2 and PINK1 proteins, male fertility as a positive regulator of androgen signaling pathway as well as cell growth and transformation through, for instance, the modulation of NF-kappa-B signaling pathway (PubMed:12612053, PubMed:15502874, PubMed:14749723, PubMed:17015834, PubMed:21097510, PubMed:18711745). Eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death (PubMed:16390825). Required for correct mitochondrial morphology and function as well as for autophagy of dysfunctional mitochondria (PubMed:19229105, PubMed:16632486). Plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking (PubMed:18711745). Regulates astrocyte inflammatory responses, may modulate lipid rafts-dependent endocytosis in astrocytes and neuronal cells (PubMed:23847046). In pancreatic islets, involved in the maintenance of mitochondrial reactive oxygen species (ROS) levels and glucose homeostasis in an age- and diet dependent manner. Protects pancreatic beta cells from cell death induced by inflammatory and cytotoxic setting. Binds to a number of mRNAs containing multiple copies of GG or CC motifs and partially inhibits their translation but dissociates following oxidative stress (PubMed:18626009). Metal-binding protein able to bind copper as well as toxic mercury ions, enhances the cell protection mechanism against induced metal toxicity (PubMed:23792957). In macrophages, interacts with the NADPH oxidase subunit NCF1 to direct NADPH oxidase-dependent ROS production, and protects against sepsis.
NCBI Summary:The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q99497
NCBI GenInfo Identifier:56404943
NCBI Gene ID:11315
NCBI Accession:Q99497.2
UniProt Secondary Accession:Q99497,O14805, Q6DR95, Q7LFU2, B2R4Z1,
UniProt Related Accession:Q99497
Molecular Weight:19,891 Da
NCBI Full Name:Protein/nucleic acid deglycase DJ-1
NCBI Synonym Full Names:Parkinsonism associated deglycase
NCBI Official Symbol:PARK7  
NCBI Official Synonym Symbols:DJ1; DJ-1; GATD2; HEL-S-67p  
NCBI Protein Information:protein/nucleic acid deglycase DJ-1
UniProt Protein Name:Protein/nucleic acid deglycase DJ-1
UniProt Synonym Protein Names:Maillard deglycase
Protein Family:DJ-1 protein
UniProt Gene Name:PARK7  
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