Pappalysin-2/PAPP-A2 is the second member of the pappalysin family of metzincin superfamily, of which PAPP-A is the first member. There is no homology between the prepro-peptides of PAPP-A and PAPP-A2, but 46% of the residues of mature PAPP-A are also present in mature PAPP-A2. PAPP-A specifically cleaves insulin-like growth factor-binding protein(IGFBP)-4, one of six known modulators of IGF-I and –II, whereas PAPP-A2 specifically cleaved IGFBP-5 at one site, between Ser-143 and Lys-144. In contrast to the cleavage of IGFBP-4 by PAPP-A that strictly requires the presence of IGF, the cleavage of IGFBP-5 by PAPP-A2 was IGF-independent. Recent data firmly establish PAPP-A and IGFBP-4 as an important functional pair in several systems. Because of its close relationship with PAPP-A, both structurally and functionally, PAPP-A2 is a likely candidate for IGFBP-5 proteinase in many tissues and conditioned media where IGFBP-5 proteolysis has been reported.
Product Name:
Human PAPPA2/Pappalysin 2 Recombinant Protein (RPES3909)
Product Code:
RPES3909
Size:
20µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
PAPP-A2,PAPP-E,PAPPE,PLAC3
Accession:
NP_064714.2
Sequence:
Ser 234-Cys 1396
Fusion tag:
C-His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human PAPPA2 mature form (NP_064714.2) corresponding to amino acid (Ser 234-Cys 1396) was expressed? with a carboxy-terminal polyhistidine tag.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
131 kDa
AP Mol Mass:
170-180 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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