Human PAPPA (Pregnancy Associated Plasma Protein A) ELISA Kit
The Human PAPPA (Pregnancy-Associated Plasma Protein A) ELISA Kit is specifically designed for the precise measurement of PAPPA levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it an excellent tool for various research applications.PAPPA is a vital protein involved in pregnancy-related processes, including fetal development and maternal health.
Abnormal levels of PAPPA have been linked to complications during pregnancy, such as preeclampsia and fetal growth restriction. Studying PAPPA levels can provide valuable insights into these conditions and aid in the development of effective diagnostic and therapeutic strategies.Get your Human PAPPA ELISA Kit today and unlock the potential to advance your research in the fields of obstetrics, gynecology, and reproductive biology.
Product Name:
Human PAPPA (Pregnancy Associated Plasma Protein A) ELISA Kit
SKU:
HUES01420
Target:
Human PAPPA (Pregnancy Associated Plasma Protein A)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PAPPA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PAPPA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PAPPA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PAPPA. You can calculate the concentration of Human PAPPA in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-100
89-101
96-110
Average (%)
95
95
102
1:4
Range (%)
98-110
86-99
96-112
Average (%)
103
91
104
1:8
Range (%)
98-111
85-97
94-107
Average (%)
105
90
100
1:16
Range (%)
96-113
84-96
92-105
Average (%)
104
91
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-96
91
EDTA plasma (n=5)
95-106
100
Cell culture media (n=5)
88-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.7
6.8
17.6
2.5
6.8
17.3
Standard deviation
0.1
0.4
0.7
0.1
0.3
0.8
C V (%)
3.7
5.88
3.98
4.0
4.41
4.62
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PAPPA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PAPPA in samples. No significant cross-reactivity or interference between Human PAPPA and analogues was observed.
