The Human PAP (Plasmin-AntiPlasmin) Complex ELISA Kit is a reliable and accurate tool designed for the detection of PAP levels in human serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit delivers consistent and reproducible results, making it suitable for various research applications.The Plasmin-AntiPlasmin complex is a key player in the fibrinolytic system, involved in the breakdown of blood clots.
Dysregulation of this system can lead to various cardiovascular diseases, highlighting the importance of studying PAP levels. This kit can be instrumental in understanding the role of PAP in thrombotic disorders and in developing potential therapeutic interventions.
Product Name:
Human PAP (Plasmin-Antiplasmin Complex) ELISA Kit
SKU:
HUES03010
Target:
Human PAP (Plasmin-Antiplasmin Complex)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PAP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PAP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PAP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PAP. You can calculate the concentration of Human PAP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-101
97-110
89-103
Average (%)
96
104
94
1:4
Range (%)
92-105
81-93
87-98
Average (%)
98
87
93
1:8
Range (%)
88-102
83-95
87-99
Average (%)
93
88
92
1:16
Range (%)
87-100
83-96
82-94
Average (%)
94
90
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-103
98
EDTA plasma (n=5)
89-100
95
Cell culture media (n=5)
89-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.56
24.76
79.81
11.29
27.08
85.82
Standard deviation
0.74
1.39
3.02
0.57
1.56
2.75
C V (%)
7.01
5.61
3.78
5.05
5.76
3.2
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PAP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PAP in samples. No significant cross-reactivity or interference between Human PAP and analogues was observed.
