The Human PAH (Phenylalanine Hydroxylase) ELISA Kit is specifically designed for the accurate and precise detection of phenylalanine hydroxylase levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and reproducible results for various research applications.Phenylalanine hydroxylase is an essential enzyme involved in the metabolism of the amino acid phenylalanine. Mutations in the PAH gene can lead to phenylketonuria (PKU), a genetic disorder that causes an accumulation of phenylalanine in the body.
Monitoring phenylalanine hydroxylase levels is crucial for the diagnosis and management of PKU and other related disorders.The Human PAH ELISA Kit provides researchers with a valuable tool for studying PKU, evaluating treatment outcomes, and developing potential therapies for individuals with PAH deficiencies. Its superior performance and ease of use make it a must-have for laboratories conducting research in the field of metabolic disorders and genetic diseases.
Product Name:
Human PAH (Phenylalanine Hydroxylase) ELISA Kit
SKU:
HUES02511
Target:
Human PAH (Phenylalanine Hydroxylase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PAH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PAH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PAH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PAH. You can calculate the concentration of Human PAH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-106
91-104
100-113
Average (%)
101
96
105
1:4
Range (%)
87-100
86-101
86-101
Average (%)
94
93
92
1:8
Range (%)
92-105
80-91
83-94
Average (%)
98
86
89
1:16
Range (%)
91-106
81-94
82-91
Average (%)
98
86
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
94
EDTA plasma (n=5)
93-108
99
Cell culture media (n=5)
92-104
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
252.26
581.22
1975.95
237.28
610.23
1869.07
Standard deviation
12.89
31.85
100.18
16.49
29.66
71.4
C V (%)
5.11
5.48
5.07
6.95
4.86
3.82
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PAH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PAH in samples. No significant cross-reactivity or interference between Human PAH and analogues was observed.
