OX40 (CD134) and its binding partner, OX40L (CD252), are members of the tumor necrosis factor receptor/tumor necrosis factor superfamily, is known to break an existing state of tolerance in malignancies, leading to a reactivation of antitumor immunity. The interaction between OX40 and OX40L plays an important role in antigen-specific T-cell expansion and survival. OX40 and OX40L also regulate cytokine production from T cells, antigen-presenting cells, natural killer cells, and natural killer T cells, and modulate cytokine receptor signaling. In line with these important modulatory functions, OX40-OX40L interactions have been found to play a central role in the development of multiple inflammatory and autoimmune diseases, making them attractive candidates for intervention in the clinic. Conversely, stimulating OX40 has shown it to be a candidate for therapeutic immunization strategies for cancer and infectious disease.Immune CheckpointImmune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: WB AntibodiesImmune Checkpoint ProteinsImmune Checkpoint Targets Co-stimulatory Immune Checkpoint Targets Immunotherapy Cancer Immunotherapy Targeted Therapy
Product Name:
Human OX40/TNFRSF4 Recombinant Protein (RPES4061)
Product Code:
RPES4061
Size:
50µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
Tumor necrosis factor receptor superfamily member 4,TNFRSF4,OX40,CD134,Txgp1,ACT35,IMD16,TXGP1L
Accession:
NP_003318.1
Sequence:
Met 1-Ala 216
Fusion tag:
C-His & Fc
Activity:
Immobilized Cynomolgus mFc-TNFSF4 at 10 µg/ml (100 ?l/well) can bind human TNFRSF4-Fch, The EC50 of human TNFRSF4-Fch is 0.23-0.55 µg/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain (Met 1-Ala 216) of human TNFRSF4 (NP_003318.1) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Purity:
> 85 % as determined by reducing SDS-PAGE.
Mol Mass:
48.2 kDa
AP Mol Mass:
68 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
