OX40;also termed CD134 and TNFRSF4; is a T cell co-stimulatory molecule of the TNF receptor superfamily which plays a key role in the survival and homeostasis of effector and memory T cells. OX40 is expressed on CD4+ and CD8+ T cells upon engagement of the TCR by antigen presenting cells along with co-stimulation by CD40-CD40 Ligand and CD28-B7. The interaction between OX40 and OX40 ligand (OX40L) will occur when activated T cells bind to professional antigen-presenting cells (APCs). The T-cell functions; including cytokine production; expansion; and survival; are then enhanced by the OX40 costimulatory signals. OX40 signals are critical for controlling the function and differentiation of Foxp3+ regulatory T cells. OX40-OX40L interaction regulates T-cell tolerance; peripheral T-cell homeostasis; and T-cell-mediated inflammatory diseases.
Product Name:
Human OX40/TNFRSF4 Recombinant Protein (RPES1023)
Product Code:
RPES1023
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Recombinant Human OX40/TNFRSF4 Protein (C-Fc)
Accession:
P43489
Sequence:
Leu29-Ala216
Fusion tag:
C-Fc
Activity:
Immobilized Human OX40L-6His(Cat: PKSH032842) at 2µg/ml(100 ?l/well) can bind Human OX40-Fc. The ED50 of Human OX40-Fc is 4.23 ug/ml .
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human OX40L Receptor is produced by our Mammalian expression system and the target gene encoding Leu29-Ala216 is expressed with a Fc tag at the C-terminus.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
46.8 kDa
AP Mol Mass:
70 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS; pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
