The Human OSM (Oncostatin M) ELISA Kit is specifically designed for the precise measurement of oncostatin M levels in human samples, including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers consistent and accurate results, making it suitable for various research applications.Oncostatin M is a potent cytokine that plays a crucial role in regulating inflammation, immune responses, and cell growth and differentiation.
It is implicated in various physiological and pathological processes, including cancer, autoimmune diseases, and inflammatory disorders, making it a valuable biomarker for studying these conditions and identifying potential therapeutic targets.Overall, the Human OSM ELISA Kit is a reliable tool for researchers seeking to uncover the role of oncostatin M in health and disease, offering a means to advance our understanding and potentially develop novel treatment strategies.
Product Name:
Human OSM (Oncostatin M) ELISA Kit
SKU:
HUES03133
Target:
Human OSM (Oncostatin M)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human OSM. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human OSM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human OSM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human OSM. You can calculate the concentration of Human OSM in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
90-104
96-114
Average (%)
99
97
104
1:4
Range (%)
91-105
85-100
86-100
Average (%)
96
92
92
1:8
Range (%)
89-100
81-94
88-101
Average (%)
95
88
94
1:16
Range (%)
89-103
85-98
83-94
Average (%)
94
91
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-108
101
EDTA plasma (n=5)
91-105
98
Cell culture media (n=5)
91-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.22
133.73
378.91
58.7
130.37
348.7
Standard deviation
3.54
5.76
15.19
3.69
5.27
11.19
C V (%)
6.53
4.31
4.01
6.29
4.04
3.21
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human OSM concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human OSM in samples. No significant cross-reactivity or interference between Human OSM and analogues was observed.
