Human OIT3 (Oncoprotein Induced Transcript 3) ELISA Kit
The Human OIT3 (Oncoprotein-Induced Transcript 3) ELISA Kit is a specialized assay designed for the accurate measurement of OIT3 levels in human samples including serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, providing reliable and reproducible results for a variety of research applications.Oncoprotein-Induced Transcript 3 (OIT3) is a protein that is implicated in various cellular processes, including cell growth and proliferation. Research has shown that OIT3 plays a role in cancer progression and development, making it a valuable biomarker for studying cancer biology and potential therapeutic interventions.
With its advanced technology and superior performance, the Human OIT3 ELISA Kit is an essential tool for researchers seeking to explore the molecular mechanisms of cancer and identify novel targets for treatment. Get reliable results and valuable insights with this cutting-edge ELISA kit from Assay Genie.
Product Name:
Human OIT3 (Oncoprotein Induced Transcript 3) ELISA Kit
SKU:
HUES02123
Target:
Human OIT3 (Oncoprotein Induced Transcript 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human OIT3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human OIT3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human OIT3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human OIT3. You can calculate the concentration of Human OIT3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-114
87-99
84-96
Average (%)
105
92
90
1:4
Range (%)
89-99
86-101
85-95
Average (%)
94
92
90
1:8
Range (%)
86-98
86-98
82-93
Average (%)
92
91
87
1:16
Range (%)
88-99
82-94
84-98
Average (%)
93
89
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
99
EDTA plasma (n=5)
94-107
101
Cell culture media (n=5)
89-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.01
2.9
6.71
1.08
2.7
6.69
Standard deviation
0.07
0.13
0.27
0.06
0.12
0.33
C V (%)
6.93
4.48
4.02
5.56
4.44
4.93
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human OIT3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human OIT3 in samples. No significant cross-reactivity or interference between Human OIT3 and analogues was observed.
