The Human OGN (Osteoglycin) ELISA Kit is a valuable tool for the precise measurement of osteoglycin levels in human samples including serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Osteoglycin is a key protein involved in regulating bone formation and remodeling, as well as influencing collagen organization.
Changes in osteoglycin levels have been linked to conditions such as osteoporosis, osteoarthritis, and cardiovascular diseases, highlighting its importance as a biomarker for studying these diseases and exploring potential treatments.Overall, the Human OGN (Osteoglycin) ELISA Kit is a reliable and effective tool for researchers seeking to investigate the role of osteoglycin in various physiological and pathological processes.
Product Name:
Human OGN (Osteoglycin) ELISA Kit
SKU:
HUES03064
Target:
Human OGN (Osteoglycin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human OGN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human OGN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human OGN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human OGN. You can calculate the concentration of Human OGN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-106
86-99
91-104
Average (%)
101
92
99
1:4
Range (%)
87-100
86-98
89-103
Average (%)
93
91
94
1:8
Range (%)
89-105
80-93
87-101
Average (%)
96
85
93
1:16
Range (%)
91-102
85-96
80-93
Average (%)
96
91
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
95
EDTA plasma (n=5)
89-104
96
Cell culture media (n=5)
96-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.16
3.48
14.57
2.3
3.5
13.57
Standard deviation
0.13
0.14
0.53
0.14
0.15
0.41
C V (%)
6.02
4.02
3.64
6.09
4.29
3.02
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human OGN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human OGN in samples. No significant cross-reactivity or interference between Human OGN and analogues was observed.
