The Human OCLN (Occludin) ELISA Kit is specifically designed for the precise measurement of occludin levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.Occludin is a key protein involved in maintaining the integrity of epithelial and endothelial cell barriers, playing a crucial role in regulating the permeability of these barriers. Dysregulation of occludin has been linked to various diseases, including inflammatory bowel disease, chronic inflammation, and autoimmune disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
Overall, the Human OCLN (Occludin) ELISA Kit provides a reliable and efficient tool for researchers looking to investigate the role of occludin in health and disease, opening up new possibilities for advancing our understanding and treatment of relevant medical conditions.
Product Name:
Human OCLN (Occludin) ELISA Kit
SKU:
HUES02170
Target:
Human OCLN (Occludin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human OCLN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human OCLN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human OCLN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human OCLN. You can calculate the concentration of Human OCLN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-113
96-109
100-116
Average (%)
105
102
106
1:4
Range (%)
93-104
88-101
81-95
Average (%)
98
93
88
1:8
Range (%)
89-104
79-92
88-98
Average (%)
96
85
93
1:16
Range (%)
92-103
84-97
85-97
Average (%)
97
90
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
94
EDTA plasma (n=5)
94-105
99
Cell culture media (n=5)
89-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.47
1.57
4.79
0.49
1.45
4.45
Standard deviation
0.03
0.09
0.23
0.03
0.07
0.2
C V (%)
6.38
5.73
4.8
6.12
4.83
4.49
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human OCLN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human OCLN in samples. No significant cross-reactivity or interference between Human OCLN and analogues was observed.
