Human OAZ1 Recombinant Protein (RPPB4149)
- SKU:
- RPPB4149
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- OAZ1
- Synonyms:
- Ornithine decarboxylase antizyme 1
- OAZ
- ODC-Az
- AZI
- Source:
- Escherichia Coli
- Uniprot:
- P54368
Description
| Product Name: | Human OAZ1 Recombinant Protein |
| Product Code: | RPPB4149 |
| Size: | 10µg |
| Species: | Human |
| Target: | OAZ1 |
| Synonyms: | Ornithine decarboxylase antizyme 1, OAZ, ODC-Az, AZI, antizyme 1, MGC138338. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The OAZ1 solution (0.25mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 1mM DTT and 30% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMVKSSLQ RILNSHCFAR EKEGDKPSAT IHASRTMPLL SLHSRGGSSS ESSRVSLHCC SNPGPGPRWC SDAPHPPLKI PGGRGNSQRD HNLSANLFYS DDRLNVTEEL TSNDKTRILN VQSRLTDAKR INWRTVLSGG SLYIEIPGGA LPEGSKDSFA VLLEFAEEQL RADHVFICFH KNREDRAALL RTFSFLGFEI VRPGHPLVPK RPDACFMAYT FERESSGEEE E |
OAZ1 is a member of the ODC antizyme family. OAZ1 is auto-regulated expressed by polyamine-enhanced translational frame shifting. OAZ1 negatively controls polyamine synthesis by increasing the negative feedback loop regulating ornithine decarboxylase (ODC) activity. The OAZ1 protein inhibits ornithine decarboxylase and speeds up its detraction.
OAZ1 Human Recombinant produced in E. coli is a single polypeptide chain containing 251 amino acids (1-228) and having a molecular mass of 27.8 kDa.OAZ1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | OAZ1: Binds to, and destabilizes, ornithine decarboxylase which is then degraded. Also inhibits cellular uptake of polyamines by inactivating the polyamine uptake transporter. SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. Belongs to the ODC antizyme family. 1 isoforms of the human protein are produced by ribosomal frameshifting. |
| UniProt Protein Details: | Protein type:Inhibitor Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: cytosol Molecular Function:protein binding; ornithine decarboxylase inhibitor activity Biological Process: transport; polyamine biosynthetic process; negative regulation of catalytic activity; positive regulation of protein catabolic process; regulation of amino acid metabolic process |
| NCBI Summary: | The protein encoded by this gene belongs to the ornithine decarboxylase antizyme family, which plays a role in cell growth and proliferation by regulating intracellular polyamine levels. Expression of antizymes requires +1 ribosomal frameshifting, which is enhanced by high levels of polyamines. Antizymes in turn bind to and inhibit ornithine decarboxylase (ODC), the key enzyme in polyamine biosynthesis; thus, completing the auto-regulatory circuit. This gene encodes antizyme 1, the first member of the antizyme family, that has broad tissue distribution, and negatively regulates intracellular polyamine levels by binding to and targeting ODC for degradation, as well as inhibiting polyamine uptake. Antizyme 1 mRNA contains two potential in-frame AUGs; and studies in rat suggest that alternative use of the two translation initiation sites results in N-terminally distinct protein isoforms with different subcellular localization. Alternatively spliced transcript variants have also been noted for this gene. [provided by RefSeq, Dec 2014] |
| UniProt Code: | P54368 |
| NCBI GenInfo Identifier: | 1709427 |
| NCBI Gene ID: | 4946 |
| NCBI Accession: | P54368.3 |
| UniProt Secondary Accession: | P54368,O43382, Q14989, Q92595, Q9UPL9, |
| UniProt Related Accession: | P54368 |
| Molecular Weight: | 228 |
| NCBI Full Name: | Ornithine decarboxylase antizyme 1 |
| NCBI Synonym Full Names: | ornithine decarboxylase antizyme 1 |
| NCBI Official Symbol: | OAZ1 |
| NCBI Official Synonym Symbols: | AZI; OAZ |
| NCBI Protein Information: | ornithine decarboxylase antizyme 1; ODC-Az |
| UniProt Protein Name: | Ornithine decarboxylase antizyme 1 |
| Protein Family: | Ornithine decarboxylase antizyme |
| UniProt Gene Name: | OAZ1 |
| UniProt Entry Name: | OAZ1_HUMAN |
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