The Human NUP62 (Nucleoporin 62kDa) CLIA Kit is a highly sensitive assay designed for the accurate quantification of NUP62 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional specificity and precision, ensuring reliable and reproducible results for a variety of research applications.NUP62 is a critical component of the nuclear pore complex, responsible for regulating the transport of molecules in and out of the nucleus. Dysregulation of NUP62 has been linked to various diseases, including cancer, autoimmune disorders, and viral infections, making it a valuable biomarker for studying these conditions and potentially identifying new therapeutic targets.
With its superior performance and ease of use, the Human NUP62 CLIA Kit is an essential tool for researchers investigating the role of NUP62 in health and disease. Order now to advance your research efforts and gain valuable insights into the mechanisms underlying these complex disorders.
Product Name:
Human NUP62 (Nucleoporin 62kDa) CLIA Kit
SKU:
HUES01245
Target:
Human NUP62 (Nucleoporin 62kDa)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NUP62. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NUP62 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NUP62, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NUP62. You can calculate the concentration of Human NUP62 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-106
94-106
98-116
Average (%)
98
100
107
1:4
Range (%)
100-114
86-101
94-109
Average (%)
107
93
100
1:8
Range (%)
86-101
90-102
102-117
Average (%)
92
96
108
1:16
Range (%)
94-106
102-114
86-98
Average (%)
101
108
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-108
99
EDTA plasma (n=5)
100-117
107
Cell culture media (n=5)
91-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
182.92
382.39
1835.88
167.33
393.53
2009.91
Standard deviation
16.19
42.75
206.54
17.23
28.77
144.91
C V (%)
8.85
11.18
11.25
10.3
7.31
7.21
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human NUP62 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NUP62 in samples. No significant cross-reactivity or interference between Human NUP62 and analogues was observed.
