Human NUDT16 Recombinant Protein (RPPB2047)

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주문
SKU:
RPPB2047
Product type:
Recombinant Protein
Size:
20ug
Species:
Human
Target:
NUDT16
Synonyms:
Nudix (nucleoside diphosphate linked moiety X)-Type Motif 16
Synonyms:
FLJ31265
Synonyms:
FLJ34034
Synonyms:
FLJ36248
Source:
Escherichia Coli
Uniprot:
Q96DE0
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Description

Product Name:Human NUDT16 Recombinant Protein
Product Code:RPPB2047
Size:20µg
Species:Human
Target:NUDT16
Synonyms:Nudix (nucleoside diphosphate linked moiety X)-Type Motif 16, FLJ31265, FLJ34034, FLJ36248, U8 snoRNA-binding protein H29K, U8 snoRNA-decapping enzyme.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:The NUDT16 protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0) 1mM DTT, 100mM NaCl and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Please avoid freeze thaw cycles
Purity:Greater than 95% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MAGARRLELG EALALGSGWR HACHALLYAP DPGMLFGRIP LRYAILMQMR FDGRLGFPGG FVDTQDRSLE DGLNRELREE LGEAAAAFRV ERTDYRSSHV GSGPRVVAHF YAKRLTLEEL LAVEAGATRA KDHGLEVLGL VRVPLYTLRD GVGGLPTFLE NSFIGSAREQ LLEALQDLGL LQSGSISGLK IPAHH

NUDT16 is nuclear nucleoside diphosphatase proteins restricted in foci in the nucleolus and nucleoplasm. NUDT16 is a RNA-decapping enzyme. It binds specifically to U8 snoRNA and can remove m7G and m227G caps from RNAs, rendering them substrates for 5’-3’ exonucleases for degradation in vivo.

NUDT16 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 215 amino acids (1-195a.a.) and having a molecular mass of 23.4kDa.NUDT16 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:NUDT16: RNA-decapping enzyme that binds specifically to U8 snoRNA. Part of the U8 snoRNP complex that is required for the accumulation of mature 5.8S and 28S rRNA. Has diphosphatase activity and removes m7G and m227G caps from U8 snoRNA. Has broad substrate specificity with manganese or cobalt as cofactor and can act on various RNA species (in vitro). Belongs to the Nudix hydrolase family.
UniProt Protein Details:

Protein type:EC 3.6.1.62; Nucleolus; Hydrolase

Chromosomal Location of Human Ortholog: 3q22.1

Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus

Molecular Function:mRNA binding; snoRNA binding; m7G(5')pppN diphosphatase activity; protein homodimerization activity; metalloexopeptidase activity; GTP binding; manganese ion binding; magnesium ion binding; cobalt ion binding

Biological Process: snoRNA catabolic process; dephosphorylation; nucleobase, nucleoside and nucleotide metabolic process; positive regulation of cell proliferation; mRNA catabolic process; proteolysis; adenosine to inosine editing; IDP catabolic process

UniProt Code:Q96DE0
NCBI GenInfo Identifier:73909074
NCBI Gene ID:131870
NCBI Accession:AAH31215.2
UniProt Secondary Accession:Q96DE0,Q96N82, B4E3B4, E9PED4, F5GYJ1,
UniProt Related Accession:Q96DE0
Molecular Weight:
NCBI Full Name:NUDT16 protein
NCBI Synonym Full Names:nudix (nucleoside diphosphate linked moiety X)-type motif 16
NCBI Official Symbol:NUDT16  
NCBI Protein Information:U8 snoRNA-decapping enzyme
UniProt Protein Name:U8 snoRNA-decapping enzyme
UniProt Synonym Protein Names:IDP phosphatase (EC:3.6.1.-); IDPase; Inosine diphosphate phosphatase; Nucleoside diphosphate-linked moiety X motif 16; Nudix motif 16; U8 snoRNA-binding protein H29K; m7GpppN-mRNA hydrolase
Protein Family:Nudix hydrolase
UniProt Gene Name:NUDT16  
UniProt Entry Name:NUD16_HUMAN
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