Human NUDT16 Recombinant Protein (RPPB2047)
- SKU:
- RPPB2047
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- NUDT16
- Synonyms:
- Nudix (nucleoside diphosphate linked moiety X)-Type Motif 16
- FLJ31265
- FLJ34034
- FLJ36248
- Source:
- Escherichia Coli
- Uniprot:
- Q96DE0
Description
| Product Name: | Human NUDT16 Recombinant Protein |
| Product Code: | RPPB2047 |
| Size: | 20µg |
| Species: | Human |
| Target: | NUDT16 |
| Synonyms: | Nudix (nucleoside diphosphate linked moiety X)-Type Motif 16, FLJ31265, FLJ34034, FLJ36248, U8 snoRNA-binding protein H29K, U8 snoRNA-decapping enzyme. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The NUDT16 protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0) 1mM DTT, 100mM NaCl and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Please avoid freeze thaw cycles |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MAGARRLELG EALALGSGWR HACHALLYAP DPGMLFGRIP LRYAILMQMR FDGRLGFPGG FVDTQDRSLE DGLNRELREE LGEAAAAFRV ERTDYRSSHV GSGPRVVAHF YAKRLTLEEL LAVEAGATRA KDHGLEVLGL VRVPLYTLRD GVGGLPTFLE NSFIGSAREQ LLEALQDLGL LQSGSISGLK IPAHH |
NUDT16 is nuclear nucleoside diphosphatase proteins restricted in foci in the nucleolus and nucleoplasm. NUDT16 is a RNA-decapping enzyme. It binds specifically to U8 snoRNA and can remove m7G and m227G caps from RNAs, rendering them substrates for 5’-3’ exonucleases for degradation in vivo.
NUDT16 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 215 amino acids (1-195a.a.) and having a molecular mass of 23.4kDa.NUDT16 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | NUDT16: RNA-decapping enzyme that binds specifically to U8 snoRNA. Part of the U8 snoRNP complex that is required for the accumulation of mature 5.8S and 28S rRNA. Has diphosphatase activity and removes m7G and m227G caps from U8 snoRNA. Has broad substrate specificity with manganese or cobalt as cofactor and can act on various RNA species (in vitro). Belongs to the Nudix hydrolase family. |
| UniProt Protein Details: | Protein type:EC 3.6.1.62; Nucleolus; Hydrolase Chromosomal Location of Human Ortholog: 3q22.1 Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus Molecular Function:mRNA binding; snoRNA binding; m7G(5')pppN diphosphatase activity; protein homodimerization activity; metalloexopeptidase activity; GTP binding; manganese ion binding; magnesium ion binding; cobalt ion binding Biological Process: snoRNA catabolic process; dephosphorylation; nucleobase, nucleoside and nucleotide metabolic process; positive regulation of cell proliferation; mRNA catabolic process; proteolysis; adenosine to inosine editing; IDP catabolic process |
| UniProt Code: | Q96DE0 |
| NCBI GenInfo Identifier: | 73909074 |
| NCBI Gene ID: | 131870 |
| NCBI Accession: | AAH31215.2 |
| UniProt Secondary Accession: | Q96DE0,Q96N82, B4E3B4, E9PED4, F5GYJ1, |
| UniProt Related Accession: | Q96DE0 |
| Molecular Weight: | |
| NCBI Full Name: | NUDT16 protein |
| NCBI Synonym Full Names: | nudix (nucleoside diphosphate linked moiety X)-type motif 16 |
| NCBI Official Symbol: | NUDT16 |
| NCBI Protein Information: | U8 snoRNA-decapping enzyme |
| UniProt Protein Name: | U8 snoRNA-decapping enzyme |
| UniProt Synonym Protein Names: | IDP phosphatase (EC:3.6.1.-); IDPase; Inosine diphosphate phosphatase; Nucleoside diphosphate-linked moiety X motif 16; Nudix motif 16; U8 snoRNA-binding protein H29K; m7GpppN-mRNA hydrolase |
| Protein Family: | Nudix hydrolase |
| UniProt Gene Name: | NUDT16 |
| UniProt Entry Name: | NUD16_HUMAN |
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