Human NTXI (Cross Linked N-telopeptide of Type I Collagen) ELISA Kit
The Human NTXI (Cross-linked N-telopeptide of Type I Collagen) ELISA Kit is specifically designed for the precise measurement of NTXI levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results for a variety of research purposes.NTXI is a key marker for bone resorption and is involved in bone turnover and remodeling processes.
Elevated levels of NTXI have been associated with various bone-related disorders, including osteoporosis and osteoarthritis, making it a valuable tool for studying these conditions and developing therapeutic interventions.With its accuracy and reliability, the Human NTXI ELISA Kit is an essential tool for researchers investigating bone health and diseases related to bone metabolism.
Product Name:
Human NTXI (Cross Linked N-telopeptide of Type I Collagen) ELISA Kit
SKU:
HUES01970
Target:
Human NTXI (Cross Linked N-telopeptide of Type I Collagen)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NTXⅠ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NTXⅠ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NTXⅠ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NTXⅠ. You can calculate the concentration of Human NTXⅠ in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-112
95-109
95-107
Average (%)
105
103
102
1:4
Range (%)
87-103
80-94
88-104
Average (%)
94
86
95
1:8
Range (%)
92-106
82-95
89-101
Average (%)
97
88
95
1:16
Range (%)
87-102
83-93
81-96
Average (%)
94
87
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
91
EDTA plasma (n=5)
87-102
93
Cell culture media (n=5)
91-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.27
30.79
98.1
8.47
30.12
97.13
Standard deviation
0.54
1.35
3.18
0.43
1.5
2.93
C V (%)
5.83
4.38
3.24
5.08
4.98
3.02
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human NTXⅠ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NTXⅠ in samples. No significant cross-reactivity or interference between Human NTXⅠ and analogues was observed.
