Human NT5E (CD73) Recombinant Protein (RPPB4128)

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SKU:
RPPB4128
Product type:
Recombinant Protein
Size:
10ug
Species:
Human
Target:
NT5E (CD73)
Synonyms:
NT
eN
NT5
NTE
Source:
Escherichia Coli
Uniprot:
P21589
Frequently bought together:

Description

Product Name:Human NT5E (CD73) Recombinant Protein
Product Code:RPPB4128
Size:10µg
Species:Human
Target:NT5E (CD73)
Synonyms:NT, eN, NT5, NTE, eNT, CD73, E5NT, 5'-nucleotidase, 5'-NT, Ecto-5'-nucleotidase, NT5E.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:NT5E (CD73) protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MWELTILHTN DVHSRLEQTS EDSSKCVNAS RCMGGVARLF TKVQQIRRAE PNVLLLDAGD QYQGTIWFTV YKGAEVAHFM NALRYDAMAL GNHEFDNGVE GLIEPLLKEA KFPILSANIK AKGPLASQIS GLYLPYKVLP VGDEVVGIVG YTSKETPFLS NPGTNLVFED EITALQPEVD KLKTLNVNKI IALGHSGFEM DKLIAQKVRG VDVVVGGHSN TFLYTGN

NT5E, also known as CD73, is a plasma membrane protein which catalyzes the conversion of extracellular nucleotides into membrane-permeable nucleosides. NT5E is functions as a determinant of lymphocyte differentiation. Defects in NT5E can cause the calcification of joints and arteries.  

NT5E (CD73) Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 247amino acids (27-252 a.a) and having a molecular mass of 27kDa. NT5E (CD73) is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 3.1.3.5; Membrane protein, integral; Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; Phosphatase (non-protein); Membrane protein, GPI anchor; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide

Chromosomal Location of Human Ortholog: 6q14-q21

Cellular Component: cell surface; membrane; cytoplasm; plasma membrane

Molecular Function:5'-nucleotidase activity; metal ion binding; nucleotide binding

Biological Process: pyrimidine nucleoside catabolic process; dephosphorylation; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; adenosine biosynthetic process; negative regulation of inflammatory response; purine nucleotide catabolic process; DNA metabolic process; purine base metabolic process; AMP catabolic process

Disease: Calcification Of Joints And Arteries

NCBI Summary:The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
UniProt Code:P21589
NCBI GenInfo Identifier:112825
NCBI Gene ID:4907
NCBI Accession:P21589.1
UniProt Related Accession:P21589
Molecular Weight:
NCBI Full Name:5'-nucleotidase
NCBI Synonym Full Names:5'-nucleotidase ecto
NCBI Official Symbol:NT5E  
NCBI Official Synonym Symbols:NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA  
NCBI Protein Information:5'-nucleotidase
UniProt Protein Name:5'-nucleotidase
UniProt Synonym Protein Names:Ecto-5'-nucleotidase; CD_antigen: CD73
UniProt Gene Name:NT5E  
UniProt Entry Name:5NTD_HUMAN

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