Human NT-ProANP (N-Terminal Pro Atrial Natriuretic Peptide) CLIA Kit
The Human NT-proANP (N-Terminal pro Atrial Natriuretic Peptide) CLIA Kit is a cutting-edge tool for accurate measurement of NT-proANP levels in human samples such as serum and plasma. This CLIA kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.NT-proANP is a biomarker that plays a crucial role in the regulation of blood pressure and fluid balance in the body.
Elevated levels of NT-proANP are associated with conditions such as heart failure, hypertension, and kidney disease, making it a valuable marker for monitoring these health conditions and assessing treatment effectiveness.This advanced CLIA kit is a valuable tool for researchers and clinicians seeking to understand the role of NT-proANP in various disease states and develop targeted therapies for improved patient outcomes.
Product Name:
Human NT-ProANP (N-Terminal Pro Atrial Natriuretic Peptide) CLIA Kit
SKU:
HUES01022
Target:
Human NT-ProANP (N-Terminal Pro Atrial Natriuretic Peptide)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NT-proANP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NT-proANP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NT-proANP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NT-proANP. You can calculate the concentration of Human NT-proANP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
91-108
89-106
Average (%)
106
98
97
1:4
Range (%)
96-110
85-98
96-107
Average (%)
103
91
101
1:8
Range (%)
100-115
100-114
96-110
Average (%)
106
106
103
1:16
Range (%)
87-101
95-110
91-106
Average (%)
94
103
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-106
98
EDTA plasma (n=5)
102-115
107
Cell culture media (n=5)
97-111
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.93
1.76
8.82
0.96
1.92
8.92
Standard deviation
0.09
0.15
0.87
0.12
0.13
0.84
C V (%)
9.68
8.52
9.86
12.5
6.77
9.42
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human NT-proANP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NT-proANP in samples. No significant cross-reactivity or interference between Human NT-proANP and analogues was observed.
