The Human NSE (Neuron-Specific Enolase) ELISA Kit is specifically designed for the quantification of NSE levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes. Neuron-Specific Enolase is a key enzyme found in neurons and neuroendocrine cells, serving as a valuable marker for neuronal damage and neurodegenerative disorders. Elevated levels of NSE have been associated with conditions like stroke, traumatic brain injury, and neuroblastoma, highlighting its importance in disease diagnosis and monitoring.
With its reliable performance and ease of use, the Human NSE ELISA Kit is an indispensable tool for scientists and researchers investigating neurologic diseases and seeking novel therapeutic approaches. Get your kit today and unlock the potential of NSE in advancing your research endeavors.
Product Name:
Human NSE (Neuron-Specific Enolase) ELISA Kit
SKU:
HUES02147
Target:
Human NSE (Neuron-Specific Enolase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.40 ng/mL
Detection range:
2.34-150 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NSE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NSE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NSE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NSE. You can calculate the concentration of Human NSE in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-105
91-104
88-102
Average (%)
100
99
95
1:4
Range (%)
97-110
81-94
94-109
Average (%)
104
88
100
1:8
Range (%)
93-107
86-98
96-112
Average (%)
101
93
104
1:16
Range (%)
98-109
88-99
96-109
Average (%)
104
94
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-108
102
EDTA plasma (n=5)
84-99
90
Cell culture media (n=5)
88-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
7.4
23.3
55.2
7.6
23.7
59.3
Standard deviation
0.4
1.1
2.2
0.5
1.2
2.7
C V (%)
5.41
4.72
3.99
6.58
5.06
4.55
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human NSE concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NSE in samples. No significant cross-reactivity or interference between Human NSE and analogues was observed.
