The Human NRG-4 (Neuregulin-4) ELISA Kit is a specialized assay designed for the precise measurement of neuregulin-4 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.Neuregulin-4 is a key signaling protein that plays a critical role in various biological processes, including cardiomyocyte function, lipid metabolism, and neuronal development.
Dysregulation of neuregulin-4 has been linked to diseases such as heart failure, obesity, and neurological disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.With its advanced technology and reliability, the Human NRG-4 ELISA Kit is an essential tool for researchers seeking to unravel the complex mechanisms involving neuregulin-4 and its implications in human health and disease.
Product Name:
Human NRG-4 (Neuregulin 4) ELISA Kit
SKU:
HUES02018
Target:
Human NRG-4 (Neuregulin 4)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NRG-4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NRG-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NRG-4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NRG-4. You can calculate the concentration of Human NRG-4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-100
91-107
100-116
Average (%)
92
98
106
1:4
Range (%)
90-105
82-92
88-102
Average (%)
96
87
94
1:8
Range (%)
89-100
83-95
90-103
Average (%)
94
90
95
1:16
Range (%)
88-101
82-95
80-93
Average (%)
95
88
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-109
101
EDTA plasma (n=5)
91-104
97
Cell culture media (n=5)
93-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.5
5.6
17.49
2.35
6.02
18.7
Standard deviation
0.13
0.24
0.6
0.16
0.27
0.8
C V (%)
5.2
4.29
3.43
6.81
4.49
4.28
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human NRG-4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NRG-4 in samples. No significant cross-reactivity or interference between Human NRG-4 and analogues was observed.
