The Human NPHN (Nephrin) ELISA Kit is specifically designed for the precise measurement of nephrin levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes. Nephrin is a key protein involved in maintaining the structure and function of the kidney's filtration barrier. Changes in nephrin levels have been linked to various kidney diseases, including diabetic nephropathy and glomerulonephritis.
By measuring nephrin levels accurately, researchers can gain valuable insights into the pathogenesis of these conditions and explore potential therapeutic interventions. With its reliable performance and broad applicability, the Human NPHN (Nephrin) ELISA Kit is an indispensable tool for studying kidney-related disorders and advancing research in the field of nephrology.
Product Name:
Human NPHN (Nephrin) ELISA Kit
SKU:
HUES02849
Target:
Human NPHN (Nephrin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NPHN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NPHN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NPHN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NPHN. You can calculate the concentration of Human NPHN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-98
92-107
94-107
Average (%)
93
99
99
1:4
Range (%)
87-100
83-94
83-98
Average (%)
94
88
90
1:8
Range (%)
88-101
83-97
83-97
Average (%)
95
90
89
1:16
Range (%)
89-104
82-93
85-98
Average (%)
95
87
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-108
101
EDTA plasma (n=5)
90-106
96
Cell culture media (n=5)
89-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.48
0.9
3.58
0.52
0.83
3.85
Standard deviation
0.03
0.05
0.19
0.03
0.03
0.18
C V (%)
6.25
5.56
5.31
5.77
3.61
4.68
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human NPHN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NPHN in samples. No significant cross-reactivity or interference between Human NPHN and analogues was observed.
