Human NOX4 (Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4) ELISA Kit
The Human NOX4 (Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4) ELISA Kit is specifically designed for the precise measurement of NOX4 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.NOX4 is a critical enzyme involved in the production of reactive oxygen species (ROS) and is implicated in various physiological and pathological processes.
Dysregulation of NOX4 is associated with oxidative stress, inflammation, and numerous diseases including cardiovascular disorders, diabetes, and cancer. Therefore, the detection of NOX4 levels can provide valuable insights into disease mechanisms and potential therapeutic targets.With its reliable performance and comprehensive applications, the Human NOX4 ELISA Kit is an essential tool for researchers studying oxidative stress-related conditions and exploring novel treatment strategies.
Product Name:
Human NOX4 (Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4) ELISA Kit
SKU:
HUES03114
Target:
Human NOX4 (Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NOX4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NOX4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NOX4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NOX4. You can calculate the concentration of Human NOX4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-103
87-100
93-108
Average (%)
98
93
99
1:4
Range (%)
88-102
86-97
84-95
Average (%)
93
92
90
1:8
Range (%)
93-104
82-97
83-95
Average (%)
99
88
90
1:16
Range (%)
87-99
85-100
88-101
Average (%)
94
92
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
94
EDTA plasma (n=5)
91-103
98
Cell culture media (n=5)
92-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.48
1.47
4.12
0.5
1.56
3.96
Standard deviation
0.03
0.07
0.2
0.03
0.07
0.21
C V (%)
6.25
4.76
4.85
6.0
4.49
5.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human NOX4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NOX4 in samples. No significant cross-reactivity or interference between Human NOX4 and analogues was observed.
