Human NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) ELISA Kit
The Human NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) ELISA Kit is specifically designed for the quantitative detection of NOS2 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.NOS2, also known as iNOS, is an enzyme that plays a key role in the production of nitric oxide, a molecule involved in various physiological and pathological processes.
Abnormal NOS2 expression has been linked to inflammatory diseases, neurodegenerative disorders, and cancer, making it a critical biomarker for studying these conditions and developing potential therapeutic interventions.With its reliable performance and ease of use, the Human NOS2/iNOS ELISA Kit is an essential tool for researchers looking to investigate the role of NOS2 in health and disease. Order yours today and unlock the potential of NOS2 research.
Product Name:
Human NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) ELISA Kit
SKU:
HUES01897
Target:
Human NOS2/iNOS (Nitric Oxide Synthase 2, Inducible)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NOS2/iNOS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NOS2/iNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NOS2/iNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NOS2/iNOS. You can calculate the concentration of Human NOS2/iNOS in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-106
96-112
89-103
Average (%)
100
103
95
1:4
Range (%)
101-118
82-94
94-105
Average (%)
108
89
100
1:8
Range (%)
100-115
82-94
97-112
Average (%)
106
87
102
1:16
Range (%)
97-107
79-91
93-106
Average (%)
102
86
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
101
EDTA plasma (n=5)
94-107
99
Cell culture media (n=5)
85-95
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
276.2
441.4
2054.0
265.8
481.5
2256.9
Standard deviation
14.1
24.7
71.9
17.3
26.5
92.5
C V (%)
5.1
5.6
3.5
6.51
5.5
4.1
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human NOS2/iNOS concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NOS2/iNOS in samples. No significant cross-reactivity or interference between Human NOS2/iNOS and analogues was observed.
