Human NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal) CLIA Kit
The Human NOS1 (nNOS) Nitric Oxide Synthase 1 Neuronal CLIA Kit is a cutting-edge assay designed to accurately measure the levels of NOS1 in human samples such as serum, plasma, and cell culture supernatants. This kit boasts superior sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.NOS1, also known as neuronal nitric oxide synthase 1, is a key enzyme involved in the production of nitric oxide, a signaling molecule with diverse roles in the body including vasodilation, neurotransmission, and immune response.
Dysregulation of NOS1 has been linked to various diseases such as neurodegenerative disorders, cardiovascular diseases, and inflammatory conditions, underscoring the importance of studying this enzyme as a potential biomarker and therapeutic target.With its advanced technology and reliable performance, the Human NOS1 Nitric Oxide Synthase 1 Neuronal CLIA Kit is an essential tool for researchers seeking to unravel the roles of NOS1 in health and disease, paving the way for new insights and potential treatments.
Product Name:
Human NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal) CLIA Kit
SKU:
HUES00468
Target:
Human NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NOS1/nNOS. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NOS1/nNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NOS1/nNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NOS1/nNOS. You can calculate the concentration of Human NOS1/nNOS in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-109
99-110
88-100
Average (%)
103
104
93
1:4
Range (%)
98-114
91-106
97-110
Average (%)
105
99
104
1:8
Range (%)
88-100
97-110
101-115
Average (%)
94
104
107
1:16
Range (%)
96-113
98-115
89-104
Average (%)
103
105
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
96
EDTA plasma (n=5)
95-106
101
Cell culture media (n=5)
83-98
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
91.67
303.12
894.45
83.5
310.25
808.92
Standard deviation
9.56
25.73
62.16
9.83
33.94
62.45
C V (%)
10.43
8.49
6.95
11.77
10.94
7.72
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human NOS1/nNOS concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NOS1/nNOS in samples. No significant cross-reactivity or interference between Human NOS1/nNOS and analogues was observed.
