Human NOB1 Recombinant Protein (RPPB4115)
- SKU:
- RPPB4115
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- NOB1
- Synonyms:
- NIN1/RPN12 Binding Protein 1 Homolog
- PSMD8BP1
- PSMD8 Binding Protein 1
- Nin One Binding Protein
- Source:
- Escherichia Coli
- Uniprot:
- Q9ULX3
Description
| Product Name: | Human NOB1 Recombinant Protein |
| Product Code: | RPPB4115 |
| Size: | 20µg |
| Species: | Human |
| Target: | NOB1 |
| Synonyms: | NIN1/RPN12 Binding Protein 1 Homolog, PSMD8BP1, PSMD8 Binding Protein 1, Nin One Binding Protein, Phosphorylation Regulatory Protein HP-10, Protein ART-4, RNA-Binding Protein NOB1, Adenocarcinoma Antigen Recognized By T Lymphocytes 4, NOB1P, MST158. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The NOB1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 80.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMAPVEHV VADAGAFLRH AALQDIGKNI YTIREVVTEI RDKATRRRLA VLPYELRFKE PLPEYVRLVT EFSKKTGDYP SLSATDIQVL ALTYQLEAEF VGVSHLKQEP QKVKVSSSIQ HPETPLHISG FHLPYKPKPP QETEKGHSAC EPENLEFSSF MFWRNPLPNI DHELQELLID RGEDVPSEEE EEEENGFEDR KDDSDDDGGG WITPSNIKQI QQELEQCDVP EDVRVGCLTT DFAMQNVLLQ MGLHVLAVNG MLIREARSYI LRCHGCFKTT SDMSRVFCSH CGNKTLKKVS VTVSDDGTLH MHFSRNPKVL NPRGLRYSLP TPKGGKYAIN PHLTEDQRFP QLRLSQKARQ KTNVFAPDYI AGVSPFVEND ISSRSATLQV RDSTLGAGRR RLNPNASRKK FVKKR |
Nob1 takes part in pre-rRNA processing and cleaves in a late cytoplasmic processing step a 20S rRNA intermediate at cleavage site D to create the mature 18S rRNA. In yeast, more than 200 protein and RNA cofactors are essential for ribosome assembly, and these are mostly conserved in eukaryotes. These factors oversee alteration and cleavage of the initial 35S precursor rRNA transcript into the mature 18S, 5.8S, and 25S rRNAs, folding of the rRNA, and binding of ribosomal proteins and 5S RNA.
NOB1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 435 amino acids (1-412) and having a molecular mass of 49.1kDa.NOB1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | NOB1P: May play a role in mRNA degradation. Belongs to the NOB1 family. |
| UniProt Protein Details: | Protein type:RNA processing Chromosomal Location of Human Ortholog: 16q22.3 Cellular Component: cytosol; nucleolar preribosome, small subunit precursor; nucleoplasm Molecular Function:endoribonuclease activity Biological Process: cleavages during rRNA processing; maturation of SSU-rRNA; rRNA processing |
| NCBI Summary: | In yeast, over 200 protein and RNA cofactors are required for ribosome assembly, and these are generally conserved in eukaryotes. These factors orchestrate modification and cleavage of the initial 35S precursor rRNA transcript into the mature 18S, 5.8S, and 25S rRNAs, folding of the rRNA, and binding of ribosomal proteins and 5S RNA. Nob1 is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA (Lamanna and Karbstein, 2009 [PubMed 19706509]).[supplied by OMIM, Nov 2010] |
| UniProt Code: | Q9ULX3 |
| NCBI GenInfo Identifier: | 74753398 |
| NCBI Gene ID: | 28987 |
| NCBI Accession: | Q9ULX3.1 |
| UniProt Secondary Accession: | Q9ULX3,Q7L6B7, Q7M4M4, Q7Z4B5, Q9NWB0, |
| UniProt Related Accession: | Q9ULX3 |
| Molecular Weight: | 46,675 Da |
| NCBI Full Name: | RNA-binding protein NOB1 |
| NCBI Synonym Full Names: | NIN1/PSMD8 binding protein 1 homolog |
| NCBI Official Symbol: | NOB1 |
| NCBI Official Synonym Symbols: | ART-4; NOB1P; MST158; MSTP158; PSMD8BP1 |
| NCBI Protein Information: | RNA-binding protein NOB1 |
| UniProt Protein Name: | RNA-binding protein NOB1 |
| UniProt Synonym Protein Names: | Phosphorylation regulatory protein HP-10; Protein ART-4 |
| Protein Family: | Endoribonuclease |
| UniProt Gene Name: | NOB1 |
| UniProt Entry Name: | NOB1_HUMAN |
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