Human NME4 Recombinant Protein (RPPB4111)
- SKU:
- RPPB4111
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- NME4
- Synonyms:
- Nucleoside diphosphate kinase mitochondrial
- Nucleoside diphosphate kinase
- mitochondrial
- NDK
- Source:
- Escherichia Coli
- Uniprot:
- O00746
Description
| Product Name: | Human NME4 Recombinant Protein |
| Product Code: | RPPB4111 |
| Size: | 10µg |
| Species: | Human |
| Target: | NME4 |
| Synonyms: | Nucleoside diphosphate kinase mitochondrial, Nucleoside diphosphate kinase, mitochondrial, NDK, NDPKD, nm23-H4, NM23D. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The NME4 solution (0.5mg/ml) contains 40% glycerol, 20mM Tris-HCl buffer (pH 8.0) and 0.2M NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MPSWTRERTL VAVKPDGVQR RLVGDVIQRF ERRGFTLVGM KMLQAPESVL AEHYQDLRRK PFYPALIRYM SSGPVVAMVW EGYNVVRASR AMIGHTDSAE AAPGTIRGDF SVHISRNVIH ASDSVEGAQR EIQLWFQSSE LVSWADGGQH SSIHPA |
| Biological Activity: | Specific activity is > 120unit/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH. |
Non-Metastatic Cells 4 or NME4, is a nucleoside diphosphate kinase located in the mitochondria, and is part of the NDK family of proteins. NME4 ais a very common enzyme that enhances transfer of gamma-phosphates, through a phosphohistidine as a intermediate, between dioxynucleoside tri- and diphosphates. NME4 is originated from the nm23 gene. NME4 has a crucial part in the creation of nucleoside triphosphates that are not ATP.
NME4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 176 amino acids (33-187a.a.) and having a molecular mass of 19.6kDa.NME4 is fused to a 21 amino acid His tag at N-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | NME4: Major role in the synthesis of nucleoside triphosphates other than ATP. Belongs to the NDK family. |
| UniProt Protein Details: | Protein type:Kinase, nucleoside diphosphate; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; EC 2.7.4.6; Kinase, other; Mitochondrial; Other group; NDK family Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: mitochondrial inner membrane; mitochondrion Molecular Function:nucleoside diphosphate kinase activity; protein binding Biological Process: nucleobase, nucleoside and nucleotide interconversion; nucleoside metabolic process; nucleoside triphosphate biosynthetic process; purine nucleotide metabolic process; pyrimidine nucleotide metabolic process; regulation of apoptosis |
| NCBI Summary: | The nucleoside diphosphate (NDP) kinases (EC 2.7.4.6) are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates. The enzymes are products of the nm23 gene family, which includes NME4 (Milon et al., 1997 [PubMed 9099850]).[supplied by OMIM, May 2008] |
| UniProt Code: | O00746 |
| NCBI GenInfo Identifier: | 2498075 |
| NCBI Gene ID: | 4833 |
| NCBI Accession: | O00746.1 |
| UniProt Secondary Accession: | O00746,Q5U0M9, A2IDD0, |
| UniProt Related Accession: | O00746 |
| Molecular Weight: | 12,979 Da |
| NCBI Full Name: | Nucleoside diphosphate kinase, mitochondrial |
| NCBI Synonym Full Names: | NME/NM23 nucleoside diphosphate kinase 4 |
| NCBI Official Symbol: | NME4 |
| NCBI Official Synonym Symbols: | NDPK-D; NM23H4; nm23-H4 |
| NCBI Protein Information: | nucleoside diphosphate kinase, mitochondrial |
| UniProt Protein Name: | Nucleoside diphosphate kinase, mitochondrial |
| UniProt Synonym Protein Names: | Nucleoside diphosphate kinase D; NDPKD; nm23-H4 |
| UniProt Gene Name: | NME4 |
| UniProt Entry Name: | NDKM_HUMAN |
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