Human NME3 Recombinant Protein (RPPB4109)
- SKU:
- RPPB4109
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- NME3
- Synonyms:
- Nucleoside diphosphate kinase 3
- c371H62
- DR-nm23
- KIAA0516
- Source:
- Escherichia Coli
- Uniprot:
- Q13232
Description
| Product Name: | Human NME3 Recombinant Protein |
| Product Code: | RPPB4109 |
| Size: | 10µg |
| Species: | Human |
| Target: | NME3 |
| Synonyms: | Nucleoside diphosphate kinase 3, c371H6.2, DR-nm23, KIAA0516, NDPK-C, NDPKC, NM23-H3, NM23H3. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The NME3 solution (0.5mg/ml) contains 50% glycerol, 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl and 2mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MERTFLAVKP DGVQRRLVGE IVRRFERKGF KLVALKLVQA SEELLREHYA ELRERPFYGR LVKYMASGPV VAMVWQGLDV VRTSRALIGA TNPADAPPGT IRGDFCIEVG KNLIHGSDSV ESARREIALW FRADELLCWE DSAGHWLYE |
| Biological Activity: | Specific activity is > 150unit/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH. |
Non-Metastatic Cells 3 or NME3 is a possible suppressor of metastasis. NME3 can be found at a lower levels in greatly metastatic cells when compared to cells that has less metastatic potential NME3 is crucial for the nucleoside triphosphates synthesis, also, in apoptosis induction & hematopoiesis. NME3 is can be expressed during early stages of myeloid differentiation of greatly purified CD34+ cells.
NME3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 169 amino acids (22-169 a.a.) and having a molecular mass of 19.1kDa.NME3 is fused to a 21 amino acid His tag at N-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | NME3: Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Probably has a role in normal hematopoiesis by inhibition of granulocyte differentiation and induction of apoptosis. Belongs to the NDK family. |
| UniProt Protein Details: | Protein type:Nucleotide Metabolism - pyrimidine; EC 2.7.4.6; Apoptosis; Nucleotide Metabolism - purine; Kinase, nucleoside diphosphate; Motility/polarity/chemotaxis; Kinase, other; Other group; NDK family Chromosomal Location of Human Ortholog: 16q13.3 Cellular Component: mitochondrion; cytosol Molecular Function:metal ion binding; nucleoside diphosphate kinase activity; ATP binding Biological Process: GTP biosynthetic process; regulation of apoptosis; CTP biosynthetic process; pyrimidine nucleotide metabolic process; apoptosis; UTP biosynthetic process; nucleoside triphosphate biosynthetic process; nucleoside diphosphate phosphorylation; purine nucleotide metabolic process |
| UniProt Code: | Q13232 |
| NCBI GenInfo Identifier: | 21264477 |
| NCBI Gene ID: | 4832 |
| NCBI Accession: | Q13232.2 |
| UniProt Secondary Accession: | Q13232,Q9BWH4, |
| UniProt Related Accession: | Q13232 |
| Molecular Weight: | 19,015 Da |
| NCBI Full Name: | Nucleoside diphosphate kinase 3 |
| NCBI Synonym Full Names: | NME/NM23 nucleoside diphosphate kinase 3 |
| NCBI Official Symbol: | NME3 |
| NCBI Official Synonym Symbols: | NDPKC; NDPK-C; NM23H3; DR-nm23; NM23-H3; c371H6.2 |
| NCBI Protein Information: | nucleoside diphosphate kinase 3; NDK 3; NDP kinase 3; NDP kinase C; non-metastatic cells 3, protein expressed in; nucleoside diphosphate kinase C |
| UniProt Protein Name: | Nucleoside diphosphate kinase 3 |
| UniProt Synonym Protein Names: | DR-nm23; Nucleoside diphosphate kinase C; NDPKC; nm23-H3 |
| Protein Family: | Nucleoside diphosphate kinase |
| UniProt Gene Name: | NME3 |
| UniProt Entry Name: | NDK3_HUMAN |
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