Human NKp46 Recombinant Protein (RPPB4101)
- SKU:
- RPPB4101
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- NKp46
- Synonyms:
- Natural cytotoxicity triggering receptor 1
- Natural killer cell p46-related protein
- hNKp46
- NK-p46
- Source:
- Escherichia Coli
- Uniprot:
- O76036
Description
| Product Name: | Human NKp46 Recombinant Protein |
| Product Code: | RPPB4101 |
| Size: | 20µg |
| Species: | Human |
| Target: | NKp46 |
| Synonyms: | Natural cytotoxicity triggering receptor 1, Natural killer cell p46-related protein, hNKp46, NK-p46, NKp46, NK cell-activating receptor, Lymphocyte antigen 94 homolog, CD335 antigen, NCR1, LY94, NCRNKp46, CD335. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | NKp46 (1mg/ml) contains phosphate buffered saline (pH7.4) & 1mM EDTA. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MQQQTLPKPF IWAEPHFMVP KEKQVTICCQ GNYGAVEYQL HFEGSLFAVD RPKPPERINKVKFYIPDMNS RMAGQYSCIY RVGELWSEPS NLLDLVVTEM YDTPTLSVHP GPEVISGEEV TFYCRLDTAT SMFLLLKEGR SSHVQRGYGK VQAEFPLGPV TTAHRGTYRX FGSYNNHAWSFPSEPVKLLV TGDIENTSLA PEDPTFSADT WGTYLLTTET GLQKDHALWD HTAQN |
A natural cytotoxicity receptor (NCR) NKp46 has been shown to represent a novel NK cell-specific molecule involved in human NK cell activation. The natural cytotoxicity receptors (NCRs) are a recently characterized family of Ig-like activation receptors that appear to be major triggering receptors in tumor cell recognition. The three known NCRs include NKp46 and NKp30, which are expressed on circulating NKcells, and NKp44, which is expressed only on activating NK cells. NKp46 has been implicated in NK cell-mediated lysis of several autologous tumor cells, pathogen-infected cell lines and mononuclear phagocytes infected with an intracellular bacterium. The Lysis of tumor cells by NK-cells involves recognition by NKp46 of heparan sulfate moieties of membrane heparan sulfate proteoglycans. Furthermore, NKp46 is a surface receptor involved in NK-cell cell death by apoptosis. NKp46 has two extracellular Ig-like domains followed by a ~40 residue stalk region, a type I transmembrane domain, and a short cytoplasmic tail. The extracellular Ig-like domain of NKp46 (22-255aa) is purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer. In addition, engagement of the antigen with the monoclonal antibody stimulates intracellular calcium levels and the synthesis of cytokines. CD59 is an NKp46 coreceptor (by physical association) together they activate cytotoxicity of human NK-cells, their engagement results in tyrosine phosphorylation of CD3-zeta chains associated with NKp46. Reduced cell surface expression of NKp46 and other NK-cell receptors is linked to the impaired NK-cell cytolytic function in viremic HIV-1 infection.
NKp46 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 235 amino acids (22-255) and having a molecular mass of 26.6kDa.NKp46 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | NCR1: Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. Belongs to the natural cytotoxicity receptor (NCR) family. 6 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Receptor, misc.; Apoptosis Chromosomal Location of Human Ortholog: 19q13.42 Cellular Component: SWI/SNF complex; integral to plasma membrane Molecular Function:receptor signaling protein activity Biological Process: regulation of natural killer cell mediated cytotoxicity; natural killer cell activation; cellular defense response; signal transduction |
| UniProt Code: | O76036 |
| NCBI GenInfo Identifier: | 47605775 |
| NCBI Gene ID: | 9437 |
| NCBI Accession: | O76036.1 |
| UniProt Secondary Accession: | O76036,O76016, O76017, O76018, B0V3L2, B0V3L3, B0V3L4 B0V3L5, B8JL03, |
| UniProt Related Accession: | O76036 |
| Molecular Weight: | 34,409 Da |
| NCBI Full Name: | Natural cytotoxicity triggering receptor 1 |
| NCBI Synonym Full Names: | natural cytotoxicity triggering receptor 1 |
| NCBI Official Symbol: | NCR1 |
| NCBI Official Synonym Symbols: | LY94; CD335; NKP46; NK-p46 |
| NCBI Protein Information: | natural cytotoxicity triggering receptor 1; NK cell-activating receptor; lymphocyte antigen 94 homolog (activating NK-receptor; NK-p46); natural killer cell p46-related protein |
| UniProt Protein Name: | Natural cytotoxicity triggering receptor 1 |
| UniProt Synonym Protein Names: | Lymphocyte antigen 94 homolog; NK cell-activating receptor; Natural killer cell p46-related protein; NK-p46; NKp46; hNKp46; CD_antigen: CD335 |
| Protein Family: | Niemann-Pick type C-related protein |
| UniProt Gene Name: | NCR1 |
| UniProt Entry Name: | NCTR1_HUMAN |
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