Human NHP2L1 Recombinant Protein (RPPB4096)

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SKU:
RPPB4096
Product type:
Recombinant Protein
Size:
10ug
Species:
Human
Target:
NHP2L1
Synonyms:
FA-1
Synonyms:
SPAG12
Synonyms:
SNRNP15-5
Synonyms:
SNU13
Source:
Escherichia Coli
Uniprot:
P55769
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Description

Product Name:Human NHP2L1 Recombinant Protein
Product Code:RPPB4096
Size:10µg
Species:Human
Target:NHP2L1
Synonyms:FA-1, SPAG12, SNRNP15-5, SNU13, 15.5K, U4/U6.U5 tri-snRNP 15.5 kDa protein, NHP2 Non-Histone Chromosome protein 2-like 1 (S. cerevisiae), Sperm Specific Antigen 1, SSFA1, NHPX, FA1, OTK27, High Mobility Group-like Nuclear Protein 2 homolog 1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:The NHP2L1 protein solution (0.25mg/1ml) is formulated in In 20 mM Tris-HCl Buffer (pH 7.5), 100 mM NaCl, and 10% Glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Please avoid freeze thaw cycles.
Purity:Greater than 95% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMTEADV NPKAYPLADA HLTKKLLDLV QQSCNYKQLR KGANEATKTL NRGISEFIVM AADAEPLEII LHLPLLCEDK NVPYVFVRSK QALGRACGVS RPVIACSVTI KEGSQLKQQI QSIQQSIERL LV

NHP2L1 is a member of the ribosomal protein L7Ae family. NHP2L1 protein limited to the nucleus, primarily focused in the dense fibrillar component of the nucleolus. NHP2L1 directly binds to the 5' stem-loop of U4 snRNA and has a vital part in the late stage of spliceosome assembly, prior to step I of splicing catalysis.

NHP2L1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 152 amino acids (1-128a.a.) and having a molecular mass of 16.7kDa.NHP2L1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:NHP2L1: Binds to the 5'-stem-loop of U4 snRNA and may play a role in the late stage of spliceosome assembly. The protein undergoes a conformational change upon RNA-binding. Belongs to the ribosomal protein L7Ae family.
UniProt Protein Details:

Protein type:RNA splicing; RNA-binding; Nucleolus; Spliceosome

Chromosomal Location of Human Ortholog: 22q13

Cellular Component: nucleoplasm; spliceosome; protein complex; nucleolus; nucleus

Molecular Function:snoRNA binding; protein binding; RNA binding

Biological Process: nuclear mRNA splicing, via spliceosome; RNA splicing; ribosome biogenesis and assembly; gene expression

NCBI Summary:Originally named because of its sequence similarity to the Saccharomyces cerevisiae NHP2 (non-histone protein 2), this protein appears to be a highly conserved nuclear protein that is a component of the [U4/U6.U5] tri-snRNP. It binds to the 5' stem-loop of U4 snRNA. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P55769
NCBI GenInfo Identifier:2500345
NCBI Gene ID:4809
NCBI Accession:P55769.3
UniProt Related Accession:P55769
Molecular Weight:Calculated: 14kDaObserved: 14kDa
NCBI Full Name:NHP2-like protein 1
NCBI Synonym Full Names:NHP2 non-histone chromosome protein 2-like 1 (S. cerevisiae)
NCBI Official Symbol:NHP2L1  
NCBI Official Synonym Symbols:FA1; FA-1; NHPX; 15.5K; OTK27; SNU13; SSFA1; SPAG12; SNRNP15-5  
NCBI Protein Information:NHP2-like protein 1; sperm specific antigen 1; U4/U6.U5 tri-snRNP 15.5 kDa protein; non-histone chromosome protein 2-like 1; [U4/U6.U5] tri-snRNP 15.5 kD RNA binding protein; small nuclear ribonucleoprotein 15.5kDa (U4/U6.U5); high mobility group-like nuclear protein 2 homolog 1
UniProt Protein Name:NHP2-like protein 1
UniProt Synonym Protein Names:High mobility group-like nuclear protein 2 homolog 1; OTK27; SNU13 homolog; hSNU13; U4/U6.U5 tri-snRNP 15.5 kDa proteinNHP2-like protein 1, N-terminally processed
UniProt Gene Name:NHP2L1  
UniProt Entry Name:NH2L1_HUMAN
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