Human NHP2L1 Recombinant Protein (RPPB4096)
- SKU:
- RPPB4096
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- NHP2L1
- Synonyms:
- FA-1
- SPAG12
- SNRNP15-5
- SNU13
- Source:
- Escherichia Coli
- Uniprot:
- P55769
Description
| Product Name: | Human NHP2L1 Recombinant Protein |
| Product Code: | RPPB4096 |
| Size: | 10µg |
| Species: | Human |
| Target: | NHP2L1 |
| Synonyms: | FA-1, SPAG12, SNRNP15-5, SNU13, 15.5K, U4/U6.U5 tri-snRNP 15.5 kDa protein, NHP2 Non-Histone Chromosome protein 2-like 1 (S. cerevisiae), Sperm Specific Antigen 1, SSFA1, NHPX, FA1, OTK27, High Mobility Group-like Nuclear Protein 2 homolog 1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The NHP2L1 protein solution (0.25mg/1ml) is formulated in In 20 mM Tris-HCl Buffer (pH 7.5), 100 mM NaCl, and 10% Glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Please avoid freeze thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMTEADV NPKAYPLADA HLTKKLLDLV QQSCNYKQLR KGANEATKTL NRGISEFIVM AADAEPLEII LHLPLLCEDK NVPYVFVRSK QALGRACGVS RPVIACSVTI KEGSQLKQQI QSIQQSIERL LV |
NHP2L1 is a member of the ribosomal protein L7Ae family. NHP2L1 protein limited to the nucleus, primarily focused in the dense fibrillar component of the nucleolus. NHP2L1 directly binds to the 5' stem-loop of U4 snRNA and has a vital part in the late stage of spliceosome assembly, prior to step I of splicing catalysis.
NHP2L1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 152 amino acids (1-128a.a.) and having a molecular mass of 16.7kDa.NHP2L1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | NHP2L1: Binds to the 5'-stem-loop of U4 snRNA and may play a role in the late stage of spliceosome assembly. The protein undergoes a conformational change upon RNA-binding. Belongs to the ribosomal protein L7Ae family. |
| UniProt Protein Details: | Protein type:RNA splicing; RNA-binding; Nucleolus; Spliceosome Chromosomal Location of Human Ortholog: 22q13 Cellular Component: nucleoplasm; spliceosome; protein complex; nucleolus; nucleus Molecular Function:snoRNA binding; protein binding; RNA binding Biological Process: nuclear mRNA splicing, via spliceosome; RNA splicing; ribosome biogenesis and assembly; gene expression |
| NCBI Summary: | Originally named because of its sequence similarity to the Saccharomyces cerevisiae NHP2 (non-histone protein 2), this protein appears to be a highly conserved nuclear protein that is a component of the [U4/U6.U5] tri-snRNP. It binds to the 5' stem-loop of U4 snRNA. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P55769 |
| NCBI GenInfo Identifier: | 2500345 |
| NCBI Gene ID: | 4809 |
| NCBI Accession: | P55769.3 |
| UniProt Related Accession: | P55769 |
| Molecular Weight: | Calculated: 14kDaObserved: 14kDa |
| NCBI Full Name: | NHP2-like protein 1 |
| NCBI Synonym Full Names: | NHP2 non-histone chromosome protein 2-like 1 (S. cerevisiae) |
| NCBI Official Symbol: | NHP2L1 |
| NCBI Official Synonym Symbols: | FA1; FA-1; NHPX; 15.5K; OTK27; SNU13; SSFA1; SPAG12; SNRNP15-5 |
| NCBI Protein Information: | NHP2-like protein 1; sperm specific antigen 1; U4/U6.U5 tri-snRNP 15.5 kDa protein; non-histone chromosome protein 2-like 1; [U4/U6.U5] tri-snRNP 15.5 kD RNA binding protein; small nuclear ribonucleoprotein 15.5kDa (U4/U6.U5); high mobility group-like nuclear protein 2 homolog 1 |
| UniProt Protein Name: | NHP2-like protein 1 |
| UniProt Synonym Protein Names: | High mobility group-like nuclear protein 2 homolog 1; OTK27; SNU13 homolog; hSNU13; U4/U6.U5 tri-snRNP 15.5 kDa proteinNHP2-like protein 1, N-terminally processed |
| UniProt Gene Name: | NHP2L1 |
| UniProt Entry Name: | NH2L1_HUMAN |
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