The Human NFkB (Nuclear Factor Kappa B) ELISA Kit is specifically designed for the precise measurement of NFkB levels in human samples including serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and accuracy, ensuring consistent and reliable results for a variety of research purposes.NFkB is a key transcription factor that plays a critical role in regulating the immune response, inflammation, and cell survival. Dysregulation of NFkB has been linked to various diseases including cancer, inflammatory disorders, and autoimmune conditions, making it a valuable biomarker for studying these diseases and developing potential therapeutic interventions.
With the Human NFkB ELISA Kit, researchers can confidently assess NFkB levels in human samples, advancing their understanding of NFkB signaling and its implications in disease pathogenesis. Trust this kit to deliver robust and reproducible data for your research needs.
Product Name:
Human NFkB (Nuclear Factor Kappa B) ELISA Kit
SKU:
HUES02429
Target:
Human NFkB (Nuclear Factor Kappa B)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NFKB-p105. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NFKB-p105 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NFKB-p105, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NFKB-p105. You can calculate the concentration of Human NFKB-p105 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-103
92-105
95-109
Average (%)
97
100
103
1:4
Range (%)
91-103
80-91
84-96
Average (%)
96
85
90
1:8
Range (%)
93-108
83-99
88-100
Average (%)
99
90
95
1:16
Range (%)
92-105
79-94
84-95
Average (%)
99
86
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-108
100
EDTA plasma (n=5)
88-100
95
Cell culture media (n=5)
96-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.47
1.48
4.59
0.5
1.44
4.39
Standard deviation
0.03
0.07
0.22
0.03
0.06
0.22
C V (%)
6.38
4.73
4.79
6.0
4.17
5.01
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human NFKB-p105 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NFKB-p105 in samples. No significant cross-reactivity or interference between Human NFKB-p105 and analogues was observed.
