Human NFATC2 Recombinant Protein (RPPB4086)

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SKU:
RPPB4086
Product type:
Recombinant Protein
Size:
5ug
Species:
Human
Target:
NFATC2
Synonyms:
Nuclear Factor Of Activated T Cells 2
Synonyms:
Nuclear Factor Of Activated T-Cells
Synonyms:
Cytoplasmic
Synonyms:
Calcineurin-Dependent 2
Source:
Sf9 Insect cells
Uniprot:
Q13469
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Description

Product Name:Human NFATC2 Recombinant Protein
Product Code:RPPB4086
Size:5µg
Species:Human
Target:NFATC2
Synonyms:Nuclear Factor Of Activated T Cells 2, Nuclear Factor Of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2, Nuclear Factor Of Activated T-Cells 2, NFAT Pre-Existing Subunit, NF-ATc2, NFAT1, NFATP, Nuclear Factor Of Activated T-Cells, Preexisting Component , Nuclear Factor Of Activated T-Cells, Cytoplasmic 2, NFAT Transcription Complex, Preexisting Component, Preexisting Nuclear Factor Of Activated T-Cells 2, T Cell Transcription Factor NFAT1, T-Cell Transcription Factor NFAT1,  NF-ATp, NFATc2.       
Source:Sf9 Insect cells
Physical Appearance:Sterile Filtered colorless solution.
Formulation:NFATC2 protein solution (0.25mg/ml) contains Phosphate Buffered Saline (pH 7.4) 40% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MPLEWPLSSQ SGSYELRIEV QPKPHHRAHY ETEGSRGAVK APTGGHPVVQ LHGYMENKPL GLQIFIGTAD ERILKPHAFY QVHRITGKTV TTTSYEKIVG NTKVLEIPLE PKNNMRATID CAGILKLRNA DIELRKGETD IGRKNTRVRL VFRVHIPESS GRIVSLQTAS NPIECSQRSA HELPMVERQD TDSCLVYGGQ QMILTGQNFT SESKVVFTEK TTDGQQIWEM EATVDKDKSQ PNMLFVEIPE YRNKHIRTPV KVNFYVINGK RKRSQPQHFT YHPVHHHHHH

NFATC2, also known as nuclear factor of activated T-cells 2, belongs to the nuclear factor of activated T cells family. NFATC2 takes a significant part in the course of T helper cell differentiation, activation, and effector function. Even though KO of an individual NFAT isoform in T cells directs to rather minor effects, T cells lacking for NFATC1 and 2 totally fail to produce T helper cell effector cytokines, for instance the interleukins IL-4 and IL-2. Moreover, NFATC2 is highly phosphorylated and kept in the cytoplasm. Next T cell receptor stimulation, dephosphorylation via calcium-activated calcineurin induces a conformational modification of NFATC2 which reveals a few nuclear localization sequences.

NFATC2 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 290 amino acids (396-678a.a.) and having a molecular mass of 33.1kDa. (Molecular size on SDS-PAGE will appear at approximately 28-40kDa). NFATC2 is expressed with a 7 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques. 

UniProt Protein Function:NFAT1: a transcription factor that plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha and GM-CSF. It is present in a phosphorylated the cytosol of unstimulated T cells. After T-cell activation, it is dephosphorylated by calcineurin and translocates to the nucleus as a part of a transcription complex. Two splice variant isoforms have been identified.
UniProt Protein Details:

Protein type:DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 20q13.2

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; ribonucleoprotein complex; nucleus; cytosol; actin cytoskeleton

Molecular Function:protein binding; DNA binding; transcription factor activity

Biological Process: response to drug; B cell receptor signaling pathway; cell migration; transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; positive regulation of B cell proliferation; cytokine production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; response to DNA damage stimulus

NCBI Summary:This gene is a member of the nuclear factor of activated T cells (NFAT) family. The product of this gene is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq, Apr 2012]
UniProt Code:Q13469
NCBI GenInfo Identifier:68846905
NCBI Gene ID:4773
NCBI Accession:Q13469.2
UniProt Secondary Accession:Q13469,Q13468, Q5TFW7, Q5TFW8, Q9NPX6, Q9NQH3, Q9UJR2 B5B2N8, B5B2N9, B5B2P0, B5B2P2, B5B2P3,
UniProt Related Accession:Q13469
Molecular Weight:901
NCBI Full Name:Nuclear factor of activated T-cells, cytoplasmic 2
NCBI Synonym Full Names:nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2
NCBI Official Symbol:NFATC2  
NCBI Official Synonym Symbols:NFAT1; NFATP  
NCBI Protein Information:nuclear factor of activated T-cells, cytoplasmic 2; NF-ATc2; NFAT pre-existing subunit; T cell transcription factor NFAT1; T-cell transcription factor NFAT1; NFAT transcription complex, preexisting component; preexisting nuclear factor of activated T-cells 2; nuclear factor of activated T-cells, preexisting component
UniProt Protein Name:Nuclear factor of activated T-cells, cytoplasmic 2
UniProt Synonym Protein Names:NFAT pre-existing subunit; NF-ATp; T-cell transcription factor NFAT1
Protein Family:NFATC2-interacting protein
UniProt Gene Name:NFATC2  
UniProt Entry Name:NFAC2_HUMAN
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