The Human NES1 (Nesfatin-1) ELISA Kit is specifically designed for the precise detection of Nesfatin-1 levels in human serum, plasma, and cell culture supernatants. This advanced kit boasts exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Nesfatin-1 is a key peptide hormone that plays a crucial role in regulating appetite, energy metabolism, and body weight. It is also involved in various physiological processes such as stress response, sleep regulation, and reproductive function.
Understanding Nesfatin-1 levels can provide valuable insights into conditions like obesity, diabetes, and eating disorders, making it a valuable biomarker for exploring these conditions and developing potential treatments.Overall, the Human NES1 (Nesfatin-1) ELISA Kit is an essential tool for researchers looking to investigate the role of Nesfatin-1 in human physiology and pathology, offering reliable and precise measurements to support their studies.
Product Name:
Human NES1 (Nesfatin 1) ELISA Kit
SKU:
HUES03244
Target:
Human NES1 (Nesfatin 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NES1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NES1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NES1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NES1. You can calculate the concentration of Human NES1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-111
85-96
98-110
Average (%)
104
91
104
1:4
Range (%)
91-107
81-92
88-101
Average (%)
99
86
93
1:8
Range (%)
92-104
82-93
88-98
Average (%)
98
87
93
1:16
Range (%)
86-98
87-100
86-101
Average (%)
93
92
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-109
100
EDTA plasma (n=5)
94-109
99
Cell culture media (n=5)
91-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.8
141.45
380.38
49.78
145.73
387.06
Standard deviation
2.87
6.86
19.86
3.35
8.39
20.2
C V (%)
5.54
4.85
5.22
6.73
5.76
5.22
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human NES1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NES1 in samples. No significant cross-reactivity or interference between Human NES1 and analogues was observed.
