Human NEDD8 Recombinant Protein (RPPB2018)
- SKU:
- RPPB2018
- Product type:
- Recombinant Protein
- Size:
- 25ug
- Species:
- Human
- Target:
- NEDD8
- Synonyms:
- Nedd-8
- FLJ43224
- MGC104393
- MGC125896
- Source:
- Escherichia Coli
- Uniprot:
- Q15843
Description
| Product Name: | Human NEDD8 Recombinant Protein |
| Product Code: | RPPB2018 |
| Size: | 25µg |
| Species: | Human |
| Target: | NEDD8 |
| Synonyms: | Nedd-8, FLJ43224, MGC104393, MGC125896, MGC125897, NEDD8, Ubiquitin-like protein Nedd8, Neddylin, Neural precursor cell expressed developmentally down-regulated protein 8. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The NEDD8 solution contains 20mM Tris pH 8.0, 50mM NaCl, 0.5mM DTT & 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MHHHHHHMKI EEGKLVIWIN GDKGYNGLAE VGKKFEKDTG IKVTVEHPDK LEEKFPQVAA TGDGPDIIFW AHDRFGGYAQ SGLLAEITPD KAFQDKLYPF TWDAVRYNGK LIAYPIAVEA LSLIYNKDLL PNPPKTWEEI PALDKELKAK GKSALMFNLQ EPYFTWPLIA ADGGYAFKYE NGKYDIKDVG VDNAGAKAGL TFLVDLIKNK HMNADTDYSI AEAAFNKGET AMTINGPWAW SNIDTSKVNY GVTVLPTFKG QPSKPFVGVL SAGINAASPN KELAKEFLEN YLLTDEGLEA VNKDKPLGAV ALKSYEEELA KDPRIAATME NAQKGEIMPN IPQMSAFWYA VRTAVINAAS GRQTVDEALK DAQTNSSSNN NNNNNNNNLG IEGRGSHMAA AEAANCIMEV SCGQAESSEK PNAEDMTSKD YYFDSYAHFG IHEEMLKDEV RTLTYRNSMF HNRHLFKDKV VLDVGSGTGILCMFAAKAGA RKVIGIECSS ISDYAVKIVK ANKLDHVVTI IKGKVEEVEL PVEKVDIIIS EWMGYCLFYE SMLNTVLHAR DKWLAPDGLI FPDRATLYVT AIEDRQYKDY KIHWWENVYG FDMSCIKDVA IKEPLVDVVD PKQLVTNACL IKEVDIYTVK VEDLTFTSPF CLQVKRNDYVHALVAYFNIE FTRCHKRTGF STSPESPYTH WKQTVFYMED YLTVKTGEEI FGTIGMRPNA KNNRDLDFTI DLDFKGQLCE LSCSTDYRMR |
NEDD8 is part of the ubiquitin family. Human NEDD8 shares 60% amino acid sequence homology to ubiquitin. The NEDD8 system is essential for the regulation of protein degradation pathways involved in cell cycle progression, morphogenesis and tumorigenesis. NEDD8 is involved in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E-1 complex UbE1c- appbp1 and linkage to the E-2 enzyme UbE2m. Attachment of NEDD8 to cullins activates their associated E-3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
NEDD8 Human Recombinant fused with 37 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 113 amino acids (1-76 a.a.) and having a molecular mass of 12.8 kDa.The NEDD8 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | NEDD8: a member of the ubiquitin family of proteins. Approximately 60% identical to ubiquitin protein. Activated by an E1-like complex, consisting of App-b1 and Uba3 and then linked to the E2-like enzyme, Ubc12. The major target protein modified by nedd8 is cullin-4a. Down-regulated during the development of brain. |
| UniProt Protein Details: | Protein type:Ubiquitin-like modifier Chromosomal Location of Human Ortholog: 14q12 Cellular Component: cytosol; nucleus Molecular Function:protein binding; ubiquitin protein ligase binding Biological Process: regulation of transcription from RNA polymerase II promoter; ubiquitin-dependent protein catabolic process; protein localization; anatomical structure morphogenesis; protein neddylation; transforming growth factor beta receptor signaling pathway; protein modification process; proteolysis; response to organic cyclic substance |
| UniProt Code: | Q15843 |
| NCBI GenInfo Identifier: | 2833270 |
| NCBI Gene ID: | 4738 |
| NCBI Accession: | Q15843.1 |
| UniProt Secondary Accession: | Q15843,Q3SXN8, Q6LES6, |
| UniProt Related Accession: | Q15843 |
| Molecular Weight: | 81 |
| NCBI Full Name: | NEDD8 |
| NCBI Synonym Full Names: | neural precursor cell expressed, developmentally down-regulated 8 |
| NCBI Official Symbol: | NEDD8 |
| NCBI Official Synonym Symbols: | NEDD-8 |
| NCBI Protein Information: | NEDD8; neddylin; ubiquitin-like protein Nedd8 |
| UniProt Protein Name: | NEDD8 |
| UniProt Synonym Protein Names: | Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; NEDD-8; Ubiquitin-like protein Nedd8 |
| Protein Family: | NEDD8 |
| UniProt Gene Name: | NEDD8 |
| UniProt Entry Name: | NEDD8_HUMAN |
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