Human NCALD Recombinant Protein (RPPB4060)

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SKU:
RPPB4060
Product type:
Recombinant Protein
Size:
20ug
Species:
Human
Target:
NCALD
Synonyms:
Rocalcin Delta
Synonyms:
MGC33870
Synonyms:
MGC74858
Synonyms:
Neurocalcin-Delta
Source:
Escherichia Coli
Uniprot:
P61601
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Description

Product Name:Human NCALD Recombinant Protein
Product Code:RPPB4060
Size:20µg
Species:Human
Target:NCALD
Synonyms:Rocalcin Delta, MGC33870, MGC74858, Neurocalcin-Delta.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:The NCALD protein solution (0.5mg/1ml) is formulated in 20 mM Tris-HCl buffer (pH8.0), 100mM NaCl, 1mM DTT and 40% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGKQNSKLRP EVMQDLLEST DFTEHEIQEW YKGFLRDCPS GHLSMEEFKK IYGNFFPYGD ASKFAEHVFR TFDANGDGTI DFREFIIALS VTSRGKLEQK LKWAFSMYDL DGNGYISKAE MLEIVQAIYK MVSSVMKMPE DESTPEKRTE KIFRQMDTNR DGKLSLEEFI RGAKSDPSIV RLLQCDPSSA GQF

NCALD belongs to the neuronal calcium sensor (NCS) family. NCALD is cytosolic at inactive calcium levels; but, high intracellular calcium levels induce a conformational transformation which exposes the myristoyl group, causing protein association with membranes and partial co-localization with the perinuclear trans-golgi network. NCALD protein is a regulator of signal transduction by G protein-coupled receptor.

NCALD produced in E.Coli is a single, non-glycosylated polypeptide chain containing 213 amino acids (1-193.a.a) and having a molecular mass of 24.4kDa. NCALD is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:NCALD: May be involved in the calcium-dependent regulation of rhodopsin phosphorylation. Binds three calcium ions. Belongs to the recoverin family.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 8q22.2

Cellular Component: clathrin coat of trans-Golgi network vesicle; intracellular; cytosol

Molecular Function:tubulin binding; protein binding; clathrin binding; calcium ion binding; actin binding; alpha-tubulin binding

Biological Process: vesicle-mediated transport; synaptic transmission; regulation of systemic arterial blood pressure; calcium-mediated signaling

NCBI Summary:This gene encodes a member of the neuronal calcium sensor (NCS) family of calcium-binding proteins. The protein contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. The protein is cytosolic at resting calcium levels; however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. The protein is thought to be a regulator of G protein-coupled receptor signal transduction. Several alternatively spliced variants of this gene have been determined, all of which encode the same protein; additional variants may exist but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
UniProt Code:P61601
NCBI GenInfo Identifier:47606440
NCBI Gene ID:83988
NCBI Accession:P61601.2
UniProt Related Accession:P61601
Molecular Weight:
NCBI Full Name:Neurocalcin-delta
NCBI Synonym Full Names:neurocalcin delta
NCBI Official Symbol:NCALD  
NCBI Protein Information:neurocalcin-delta
UniProt Protein Name:Neurocalcin-delta
Protein Family:Neurocalcin
UniProt Gene Name:NCALD  
UniProt Entry Name:NCALD_HUMAN
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