The Dan (Differential screening-selected gene aberrative in neuroblastoma; also known as N03) gene was first identified as the putative rat tumor suppressor gene and encodes a protein structurally related to Cerberus and Gremlin in vertebrates. It is a founding member of the DAN family of secreted proteins; acts as an inhibitor of cell cycle progression and is closely involved in retinoic acid-induced neuroblastoma differentiation. There are at least five mammalian protein members in the evolutionarily conserved Dan family including DAN; Gremlin/DRM; Cer1 (Cerberus-related); Dante and PRDC (protein related to DAN and cereberus); and share the C-terminal cystine-knot motif. As a secreted glycoprotein; DAN is a member of a class of glycoproteins shown to be secreted inhibitors of the transforming growth factor-beta (TGF-beta) and bone morphogenic protein pathways. It binds to BMPs and preventing their interactions with signaling receptor complexes; and accordingly regulates the processes of embryonic development and tissue differentiation. DAN gene product may have an important role in regulation of the entry of cells into the S phase. In addition; DAN gene product possesses an ability to revert phenotypes of transformed rat fibroblasts and represents a candidate tumour suppressor gene for neuroblastoma.
Product Name:
Human NBL1/DAND1 Recombinant Protein (RPES0116)
Product Code:
RPES0116
Size:
50µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
D1S1733E,DAN,DAND1,NB,NO3
Accession:
NP_005371.1
Sequence:
Met 1-Asp 180
Fusion tag:
C-His
Activity:
Measured by its ability to inhibit BMP4-induced activity in MC3T3-E1 Mouse osteoblastic cells. The ED50 for this effect is typically 5-20 µg/ml in the presence of 50 ng/mL of recombinant human BMP4.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human DAN precursor (NP_005371.1) (Met 1-Asp 180) was expressed with a C-terminal polyhistidine tag.
Purity:
> 98 % as determined by reducing SDS-PAGE.
Mol Mass:
19.3 kDa
AP Mol Mass:
27 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
