Myeloperoxidase (MPO) is a heme-containingenzyme belonging to the XPO subfamily of peroxidases. It is an abundant neutrophil and monocyte glycoprotein thatcatalyzes the hydrogen peroxide-dependentconversion of chloride, bromide, and iodide to multiple reactive species. Post-translationalprocessing of MPO involvesthe insertion of a heme moiety and the proteolytic removal of both a propeptide and a 6 aa internal peptide. This results in a disulfide-linkeddimer composed of a60 kDa heavy and 12 kDa light chain that associate into a 150 kDa enzymatically active tetramer. The tetramer contains two heme groups and one disulfide bondbetween the heavy chains. Alternate splicing generates two additional isoforms of MPO, one with a 32 aa insertion in the light chain, and another with a deletion ofthe signal sequence and part of the propeptide. Human and mouse MPO share 87% aa sequence identity. MPO activity results in protein nitrosylation and theformation of 3-chlorotyrosineand dityrosine crosslinks. MPO is also associated with a variety of other diseases, and inhibits vasodilation in inflammation by depleting the levelsof NO. Serum albumin functions as a carrier protein during MPO movement to the basolateral side of epithelial cells. MPO is stored in neutrophil azurophilicgranules. Upon cellular activation, it is deposited into pathogen-containingphagosomes.
Product Name:
Human Myeloperoxidase/MPO Recombinant Protein (RPES5028)
Product Code:
RPES5028
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Myeloperoxidase, MPO
Accession:
P05164
Sequence:
Ala49-Ser745
Fusion tag:
C-10His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Myeloperoxidase is produced by our Mammalian expression system and the target gene encoding Ala49-Ser745 is expressed with a 10His tag at the C-terminus.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
80.3 kDa
AP Mol Mass:
85-95 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM Tris, 150mM NaCl, pH8.0.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
