Human MYD88 (Myeloid Differentiation Factor 88) CLIA Kit
The Human MyD88 (Myeloid Differentiation Factor 88) CLIA Kit is a powerful tool for the precise quantification of MyD88 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and accuracy, guaranteeing dependable and consistent results for various research purposes.MyD88 is a critical adaptor protein involved in the signaling pathways of the innate immune system, playing a crucial role in the activation of inflammatory responses and immune defense mechanisms.
Dysregulation of MyD88 has been linked to various inflammatory diseases, autoimmune disorders, and infections, highlighting its significance as a biomarker in disease research and therapeutic development.With the Human MyD88 CLIA Kit, researchers can investigate the role of MyD88 in immune responses, inflammation, and disease pathogenesis with confidence and precision. Its user-friendly format and robust performance make it an invaluable asset in advancing scientific understanding and clinical progress.
Product Name:
Human MYD88 (Myeloid Differentiation Factor 88) CLIA Kit
SKU:
HUES01072
Target:
Human MYD88 (Myeloid Differentiation Factor 88)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MYD88. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MYD88 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MYD88, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MYD88. You can calculate the concentration of Human MYD88 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-101
100-115
101-117
Average (%)
95
108
108
1:4
Range (%)
96-111
97-111
91-104
Average (%)
102
105
97
1:8
Range (%)
87-99
85-97
89-103
Average (%)
92
91
96
1:16
Range (%)
86-99
87-103
101-117
Average (%)
92
94
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
92
EDTA plasma (n=5)
89-103
95
Cell culture media (n=5)
98-114
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
104.53
248.23
748.33
95.07
270.56
770.56
Standard deviation
10.89
24.18
79.1
8.08
23.32
75.44
C V (%)
10.42
9.74
10.57
8.5
8.62
9.79
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MYD88 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MYD88 in samples. No significant cross-reactivity or interference between Human MYD88 and analogues was observed.
