The Human MUC5B (Mucin 5 Subtype B) CLIA Kit is a reliable and accurate tool for the detection of MUC5B levels in human biological samples. This kit is optimized for use with serum, plasma, and cell culture supernatants, providing researchers with high sensitivity and specificity for consistent and reproducible results.MUC5B is a key mucin protein subtype that plays a critical role in maintaining mucosal homeostasis and protecting against pathogens in the respiratory tract. Dysregulation of MUC5B expression has been linked to various respiratory diseases such as chronic obstructive pulmonary disease (COPD), asthma, and cystic fibrosis, making it a valuable biomarker for studying these conditions and developing targeted therapies.
With the Human MUC5B CLIA Kit, researchers can accurately measure MUC5B levels in human samples, advancing our understanding of respiratory diseases and potentially leading to new diagnostic and therapeutic strategies.
Product Name:
Human MUC5B (Mucin 5 Subtype B) CLIA Kit
SKU:
HUES01188
Target:
Human MUC5B (Mucin 5 Subtype B)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MUC5B. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MUC5B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MUC5B, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MUC5B. You can calculate the concentration of Human MUC5B in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-113
95-112
103-116
Average (%)
104
102
110
1:4
Range (%)
95-110
89-104
94-108
Average (%)
101
97
102
1:8
Range (%)
85-95
90-103
87-98
Average (%)
90
98
92
1:16
Range (%)
100-117
101-116
90-104
Average (%)
107
106
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
92
EDTA plasma (n=5)
89-101
94
Cell culture media (n=5)
92-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
373.5
1161.5
3146.62
354.73
1164.49
2991.28
Standard deviation
33.88
100.93
200.75
38.42
117.85
314.08
C V (%)
9.07
8.69
6.38
10.83
10.12
10.5
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MUC5B concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MUC5B in samples. No significant cross-reactivity or interference between Human MUC5B and analogues was observed.
