The Human MUC2 (Mucin 2) ELISA Kit is specifically designed for the accurate measurement of Mucin 2 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.Mucin 2 is a key component of the mucus layer that lines the gastrointestinal tract, playing a critical role in protecting the mucosal barrier and maintaining gut health. Dysregulation of Mucin 2 expression has been associated with various gastrointestinal disorders, including inflammatory bowel disease, colorectal cancer, and mucosal infections.
As such, Mucin 2 serves as an important biomarker for understanding these conditions and exploring potential therapeutic interventions.Overall, the Human MUC2 ELISA Kit provides researchers with a valuable tool for investigating the role of Mucin 2 in gastrointestinal health and disease, offering insights into potential diagnostic and treatment strategies.
Product Name:
Human MUC2 (Mucin 2) ELISA Kit
SKU:
HUES01795
Target:
Human MUC2 (Mucin 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MUC2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MUC2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MUC2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MUC2. You can calculate the concentration of Human MUC2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-102
86-99
89-106
Average (%)
97
91
96
1:4
Range (%)
90-104
84-95
88-99
Average (%)
96
89
94
1:8
Range (%)
91-108
87-100
88-103
Average (%)
99
92
94
1:16
Range (%)
90-104
83-94
84-96
Average (%)
95
89
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
93
EDTA plasma (n=5)
93-106
100
Cell culture media (n=5)
92-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.97
2.92
7.41
1.0
3.2
7.38
Standard deviation
0.06
0.13
0.23
0.06
0.16
0.3
C V (%)
6.19
4.45
3.1
6.0
5.0
4.07
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human MUC2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MUC2 in samples. No significant cross-reactivity or interference between Human MUC2 and analogues was observed.
