Human mTOR (Mammalian Target of Rapamycin) ELISA Kit
The Human mTOR (Mammalian Target of Rapamycin) ELISA Kit is specifically designed for the accurate detection of mTOR levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.mTOR is a key protein involved in regulating cell growth, metabolism, and survival, making it a critical target for studying diseases such as cancer, diabetes, and neurological disorders.
By accurately measuring mTOR levels, researchers can gain valuable insights into disease mechanisms and potentially identify new therapeutic targets.Overall, the Human mTOR ELISA Kit from Assay Genie provides researchers with a reliable tool to investigate the role of mTOR in various physiological and pathological processes, paving the way for novel discoveries and advancements in biomedical research.
Product Name:
Human mTOR (Mammalian Target of Rapamycin) ELISA Kit
SKU:
HUES02655
Target:
Human mTOR (Mammalian Target of Rapamycin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human mTOR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human mTOR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human mTOR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human mTOR. You can calculate the concentration of Human mTOR in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-112
85-97
90-106
Average (%)
102
90
96
1:4
Range (%)
90-106
85-99
84-96
Average (%)
97
90
91
1:8
Range (%)
85-97
86-99
82-96
Average (%)
92
91
88
1:16
Range (%)
89-99
88-101
80-95
Average (%)
94
93
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-101
92
EDTA plasma (n=5)
85-99
91
Cell culture media (n=5)
90-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.49
1.42
4.3
0.46
1.42
4.44
Standard deviation
0.03
0.06
0.13
0.03
0.08
0.18
C V (%)
6.12
4.23
3.02
6.52
5.63
4.05
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human mTOR concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human mTOR in samples. No significant cross-reactivity or interference between Human mTOR and analogues was observed.
