The Human MRC1 (Mannose Receptor C Type 1) CLIA Kit is a powerful tool for detecting levels of MRC1 in human samples such as serum, plasma, and cell culture supernatants. This kit offers excellent sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.MRC1, also known as the mannose receptor, is an important protein involved in the immune response and cell adhesion. It plays a key role in recognizing and binding to foreign pathogens, facilitating their clearance from the body. Dysregulation of MRC1 has been linked to various diseases, including infectious diseases, inflammatory disorders, and cancer, highlighting its significance as a potential biomarker for disease detection and treatment.
With the Human MRC1 CLIA Kit, researchers can explore the role of MRC1 in different conditions and advance their understanding of its therapeutic potential. This kit is a valuable asset for studying immune responses, inflammation, and disease pathogenesis, providing valuable insights for biomedical research and drug development efforts.
Product Name:
Human MRC1 (Mannose Receptor C Type 1 ) CLIA Kit
SKU:
HUES01239
Target:
Human MRC1 (Mannose Receptor C Type 1 )
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MRC1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MRC1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MRC1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MRC1. You can calculate the concentration of Human MRC1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-114
91-106
100-118
Average (%)
104
97
109
1:4
Range (%)
95-108
101-113
96-111
Average (%)
102
107
102
1:8
Range (%)
94-109
89-100
84-98
Average (%)
102
95
90
1:16
Range (%)
97-112
94-104
94-108
Average (%)
104
99
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-98
93
EDTA plasma (n=5)
97-112
105
Cell culture media (n=5)
91-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
207.64
402.37
1905.92
194.26
411.68
1807.56
Standard deviation
20.87
35.69
114.55
18.42
45.37
157.26
C V (%)
10.05
8.87
6.01
9.48
11.02
8.7
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MRC1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MRC1 in samples. No significant cross-reactivity or interference between Human MRC1 and analogues was observed.
