Human MMP14 Recombinant Protein (RPPB1974)
- SKU:
- RPPB1974
- Product type:
- Recombinant Protein
- Size:
- 5ug
- Species:
- Human
- Target:
- MMP14
- Synonyms:
- MatrixMetallopeptidase 14
- Matrix Metallopeptidase 14 (Membrane-Inserted)
- Membrane-Type-1Matrix Metalloproteinase
- Membrane Type 1 Metalloprotease
- Source:
- Sf9 Insect cells
- Uniprot:
- P50281
Description
| Product Name: | Human MMP14 Recombinant Protein |
| Product Code: | RPPB1974 |
| Size: | 5µg |
| Species: | Human |
| Target: | MMP14 |
| Synonyms: | MatrixMetallopeptidase 14, Matrix Metallopeptidase 14 (Membrane-Inserted), Membrane-Type-1Matrix Metalloproteinase, Membrane Type 1 Metalloprotease, EC 3.4.24.80, MT-MMP1, MT1-MMP, MMP-14, MMP-X1, MT1MMP, MTMMP1, Matrix Metalloproteinase 14(Membrane-Inserted), Membrane-Type Matrix Metalloproteinase 1, MatrixMetalloproteinase-14, EC 3.4.24, MT-MMP, WNCHRS, Matrix metalloproteinase-14, Membrane-typematrix metalloproteinase 1. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | MMP14protein solution (0.25mg/ml) containing Phosphate Buffered Saline (pH 7.4) and10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | ADLALASLGSAQSSSFSPEA WLQQYGYLPP GDLRTHTQRS PQSLSAAIAA MQKFYGLQVT GKADADTMKA MRRPRCGVPDKFGAEIKANV RRKRYAIQGL KWQHNEITFC IQNYTPKVGE YATYEAIRKA FRVWESATPL RFREVPYAYIREGHEKQADI MIFFAEGFHG DSTPFDGEGGFLAHAYFPGP NIGGDTHFDS AEPWTVRNED LNGNDIFLVA VHELGHALGLEHSSDPSAIM APFYQWMDTE NFVLPDDDRR GIQQLYGGES GFPTKMPPQP RTTSRPSVPD KPKNPTYGPNICDGNFDTVA MLRGEMFVFK ERWFWRVRNN QVMDGYPMPI GQFWRGLPAS INTAYERKDGKFVFFKGDKH WVFDEASLEP GYPKHIKELG RGLPTDKIDA ALFWMPNGKTYFFRGNKYYR FNEELRAVDS EYPKNIKVWE GIPESPRGSF MGSDEVFTYF YKGNKYWKFN NQKLKVEPGYPKSALRDWMG CPSGGRPDEG TEEETEVIII EVDEEGGGAV SHHHHHH |
Matrix metalloproteinase-14 (MMP14), is a membrane-anchored zinc-binding endopeptidase which is expressed at the leading edge of differentinvasive carcinomas and also promotes tumor cell invasion through degradationof the extracellular matrix. MMP14 takesa vital part in extracellular matrix, ECM, remodeling by having the capabilityto degrade type I collagen, activate pro-MMP-2 and process cell adhesionmolecules for instance CD44 and integrin alpha V. MMP14 is a key enzyme in manyphysiological as well as pathological processes for example angiogenesis &tumor invasion.
MMP14 Human Recombinant produced in Sf9 Baculovirus cells is a single, non-glycosylated polypeptide chain containing 527 amino acids (21-538a.a) and having a molecular mass of 59.9kDa (Molecular size on SDS-PAGE will appear at approximately 35-70kDa). MMP14 is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | MMP14: Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Up-regulated by NANOS1. Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors. Belongs to the peptidase M10A family. |
| UniProt Protein Details: | Protein type:EC 3.4.24.80; Protease; Motility/polarity/chemotaxis; Membrane protein, integral Chromosomal Location of Human Ortholog: 14q11.2 Cellular Component: extracellular matrix; focal adhesion; Golgi lumen; integral to plasma membrane; cytoplasm; melanosome; plasma membrane Molecular Function:integrin binding; protein binding; protease activator activity; zinc ion binding; metalloendopeptidase activity; calcium ion binding; transcription factor activity Biological Process: extracellular matrix organization and biogenesis; tissue remodeling; negative regulation of focal adhesion formation; male gonad development; embryonic cranial skeleton morphogenesis; proteolysis; positive regulation of cell growth; collagen catabolic process; extracellular matrix disassembly; regulation of transcription, DNA-dependent; branching morphogenesis of a tube; ovarian follicle development; response to estrogen stimulus; response to mechanical stimulus; endothelial cell proliferation; response to hypoxia; zymogen activation; response to oxidative stress; angiogenesis; astrocyte cell migration; lung development; positive regulation of cell migration; endochondral ossification Disease: Winchester Syndrome |
| NCBI Summary: | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P50281 |
| NCBI GenInfo Identifier: | 317373419 |
| NCBI Gene ID: | 4323 |
| NCBI Accession: | P50281.3 |
| UniProt Secondary Accession: | P50281,Q6GSF3, Q92678, A8K5L0, |
| UniProt Related Accession: | P50281 |
| Molecular Weight: | 65,894 Da |
| NCBI Full Name: | Matrix metalloproteinase-14 |
| NCBI Synonym Full Names: | matrix metallopeptidase 14 (membrane-inserted) |
| NCBI Official Symbol: | MMP14 |
| NCBI Official Synonym Symbols: | MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; WNCHRS; MT1-MMP; MT-MMP 1 |
| NCBI Protein Information: | matrix metalloproteinase-14; membrane type 1 metalloprotease; membrane-type-1 matrix metalloproteinase |
| UniProt Protein Name: | Matrix metalloproteinase-14 |
| UniProt Synonym Protein Names: | MMP-X1; Membrane-type matrix metalloproteinase 1; MT-MMP 1; MTMMP1; Membrane-type-1 matrix metalloproteinase; MT1-MMP; MT1MMP |
| Protein Family: | Matrix metalloproteinase |
| UniProt Gene Name: | MMP14 |
| UniProt Entry Name: | MMP14_HUMAN |
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